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| Apogossypolone诱导前列腺癌PC-3细胞在体外的自噬 | |
| 引用本文： | 袁青,陈晓鹏,黄晓峰,穆士杰,胡兴斌,尹文,张献清. Apogossypolone诱导前列腺癌PC-3细胞在体外的自噬[J]. 肿瘤防治研究, 2011, 38(9): 1006-1011. DOI: 10.3971/j.issn.1000-8578.2011.09.009 |
| 作者姓名： | 袁青 陈晓鹏 黄晓峰 穆士杰 胡兴斌 尹文 张献清 |
| 作者单位： | 1.710032 西安，第四军医大学西京医院急诊科, 2.输血科；3.第四军医大学基础医学部中心实验室 |
| 摘 要： | 目的研究ApoG2对前列腺癌PC-3细胞在体外的作用，了解其杀伤肿瘤细胞的机制。方法采用MTT法、吖啶橙染色、透射电镜、流式细胞技术、Western blot、免疫组织化学等方法观察了ApoG2对PC-3细胞的自噬与凋亡的诱导作用。结果ApoG2可明显抑制PC-3细胞增殖；ApoG2作用于PC-3细胞72小时可诱导细胞自噬；加入自噬抑制剂3-MA可增强ApoG2诱导凋亡作用；ApoG2可以增强细胞内LC-3Ⅱ及Beclin-Ⅰ的表达,降低Bcl-2的表达水平。结论ApoG2主要以诱导PC-3细胞发生自噬为主，抑制自噬可以促进凋亡的发生。 |
| 关 键 词： | ApoG2 人前列腺癌PC-3细胞 自噬 凋亡 |
| 收稿时间： | 2010-10-19; |
Apogossypolone Induces Autophagy of PC-3 Prostate Cancer Cells in vitro |
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| YUAN Qing,CHEN Xiao-peng,HUANG Xiao-feng,MU Shi-jie,HU Xing-bin,YIN Wen,ZHANG Xian-qing. Apogossypolone Induces Autophagy of PC-3 Prostate Cancer Cells in vitro[J]. Cancer Research on Prevention and Treatment, 2011, 38(9): 1006-1011. DOI: 10.3971/j.issn.1000-8578.2011.09.009 | |
| Authors: | YUAN Qing CHEN Xiao-peng HUANG Xiao-feng MU Shi-jie HU Xing-bin YIN Wen ZHANG Xian-qing |
| Affiliation: | 1.Emergency Department，Xijing Hospital,Fourth Military Medical University,Xi’an 710032,China， 2.Department of Blood Transfusion; 3.Central Laboratory,Department of Basic Medicine,Fourth Military Medical University |
| Abstract: | Objective To study the growth inhibitation action of gossypol derivatives,ApoG2 on the prostatic cancer PC-3 cells,and make the primary study about their anti-tumor mechanism.Methods The MTT analytical method,AO staining,transmission electron microscope,flow cytometer and Western blot for measuring the apoptosis related proteins were used to study the growth inhibition and induction of autophagy roles of ApoG2 on the prostatic cancer PC-3 cells in vitro.Results When the concentration of ApoG2 was higher tha... |
| Keywords: | Apogossypolone Prostate cancer PC-3 cells Autophagy Apoptosis |
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