应用MRI和电镜观察pSVPoMcat微基因修饰雪旺氏细胞移植对损伤脊髓的修复作用

目的：探讨ｐＳＶＰｏＭｃａｔ微基因修饰雪旺氏细胞（ＳＣ）对损伤脊髓再生修复的作用。方法：采用切割法制备健康ＳＤ大鼠脊髓半横断损伤模型,随机植入ｐＳＶＰｏＭｃａｔ微基因修饰的ＳＣ（Ａ组）,高纯化的ＳＣ（Ｂ组）,仅植入明胶海绵的损伤对照组（Ｃ组）。受体鼠存活３月,行ＭＲＩ扫描,取材作电镜（ＴＥＭ）观察。结果：ＭＲＩ发现：Ａ组动物,脊髓损伤（ＳＣＩ）区脊髓信号已基本恢复正常,Ｂ组动物未恢复正常,而Ｃ组动

MRI AND ULTRASTUCTURAL OBSERVATION OF THE REPAIRING EFFECTS OF TRANSPLANTED pSVPoMcat GENETICALLY MODIFIED SCHWANN CELLS IN INJURIED SPINAL CORD IN RATS

Objective Toapproach the effect of genetically modified Schwann cell (SC) with pSVPoMcat in theregeneration of spinal cord injury (SCI). Methods Experimental animal was divided intothree groups the pSVPoMcat genetically mod ified SC implantation group (A group), SCimplantation group (B group), and cont rol group (C group). After three month the presenceof axonal regeneration of th e injuried spinal cord was examined by means of MRI and TEM.Results [ WTBZ The result s were found (1) The MRI spinal cord signal of SCI region hasbasic recovered i n A group and has not recoved completely in B group. There was a malaciafocus i n C group. (2) The outcome of TBM observation in A group, B group was purification SC and regenerated axons, necrotic SC partially and regenerated axons, respec tively.Otherwise, there were not regenerated axons in C group. Conclusi ons Thi s result isconsidered to promote axonal regeneration and potential for further clinical application.