| siRNA下调Caveolin-1表达对体外培养C6胶质瘤细胞影响 |
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| 引用本文： | 李飞,李梅,卢佳友,朱刚,林江凯,孟辉,吴南,陈志,冯华. siRNA下调Caveolin-1表达对体外培养C6胶质瘤细胞影响[J]. 中华神经外科杂志, 2010, 26(2). DOI: 10.3760/cma.j.issn.1001-2346.2010.02.025 |
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| 作者姓名： | 李飞 李梅 卢佳友 朱刚 林江凯 孟辉 吴南 陈志 冯华 |
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| 作者单位： | 第三军医大学西南医院神经外科,重庆,400038 |
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| 基金项目： | 国家自然科学基金，国家科技支撑计划，第三军医大学科研创新基金 |
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| 摘 要： | 目的 观察siRNA下调Caveolin-1(Car1)表达的效果及对体外培养C6胶质瘤细胞侵袭和迁移能力的影响.方法 构建Car1 mRNA质粒,转染体外培养c6胶质瘤细胞,利用Transwell体外侵袭实验和"划痕"实验分别检测细胞的侵袭和迁移能力,采用Western blot和RT-PCR的方法检测Cav1、EGFR、Pyk2的表达情况.结果 所构建的Cav1 siRNA质粒转染体外培养C6胶质瘤细胞可明显下调Cav1的表达,干扰成功后C6胶质瘤细胞的侵袭能力和迁移能力明显下降,同时EGFR和Pyk2的表达明显减少.结论 siRNA下调Cav1可降低体外培养C6胶质瘤细胞的侵袭和迁移能力.
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| 关 键 词： | 神经胶质瘤 大鼠 RNA,小分子干扰 |
Effects of knock -down Caveolin -1 by siRNA on in vitro migration and invasion of C6 glioma cell |
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| LI Fei,LI Mei,LU Jia-you,ZHU Gang,LIN Jiang-kai,MENG Hui,WU Nan,CHEN Zhi,FENG Hua. Effects of knock -down Caveolin -1 by siRNA on in vitro migration and invasion of C6 glioma cell[J]. Chinese Journal of Neurosurgery, 2010, 26(2). DOI: 10.3760/cma.j.issn.1001-2346.2010.02.025 |
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| Authors: | LI Fei LI Mei LU Jia-you ZHU Gang LIN Jiang-kai MENG Hui WU Nan CHEN Zhi FENG Hua |
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| Abstract: | Objective To study the in vitro migration and invasion of C6 glioma cell after Caveolin -1 (Car1) knocking- down by siRNA. Methods Car1 siRNA plasmids were transfected to C6 glioma cells. Abilities of cell invasion and migration were examined by Transwell and "Wound - healing" methods respectively. Expressions of eaveolin-1, EGFR, Pyk2 were measured by Western blot and RT - PCR. Results The siRNA plasmid used in this experiment can obviously knock - down the expression of Car1. The invasion and the migration ability of C6 glioma cell decreased after siRNA as well as the expression of EGFR and Pyk2. Conclusions Carl Knocking - down results in the decreasing of in vitro invasion and migration of C6 glioma. It maybe a potential target for anti - invasion therapy on gliomas. |
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| Keywords: | Caveolin-1 |
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