复方三根制剂配合瘤内注射DDP调控荷肺癌小鼠Bcl-2、P53基因表达的实验研究

目的：观察与探讨Bcl-2及P53基因在荷肺癌小鼠经复方三根制剂配合瘤内注射治疗后的表达情况。方法：用lewis肺癌瘤株造模于38只C57BL／6近交系小鼠右腋下，8天后将其随机分成2组：A组（13只）、B组（12只）、C组（13只），并分别予以A组：复方三根制剂加瘤内注射DDP治疗，B组：单纯DDP瘤内注射治疗，C组：瘤内注射NS对照。21天后处死所有小鼠并取下肿瘤标本作HE染色及免疫组化染色检测，观察Bcl-2、P53表达情况。结果：Bcl-2表达情况：A组为46．1％，B组为58．3％，C组为61．5％；P53表达情况：A组为38．4％，B组为41．67％，C组69．2％。结论：瘤内注射DDP可以通过降低Bcl-2、MTP53表达途径促进肿瘤细胞凋亡，而中药复方三根制荆能协同DDP进一步下调两者的表达。

Research on the Expressions of Gene Bcl-2 and P53 in Mice Bearing Lewis Lung Cancer After Administered with DDP by Intratumor Injection and FFSG Mixture by Oral

Objective:To observe the statement of gene Bcl-2 and P53 in mice bearing lewis lung cancer after administered DDP by intratumor injection and FFSG mixture by oral.Methods:To prepare model mice of c57b1/6 planted lewis lung cancer.The models were randomly divided into three groups, marked group A, group B and C in 8 days.Two groups were treated with DDP by intratumor injection and FFSG mixture by oral and simply administered DDP by intratumor injection respectively.Group C was administered DDP by intratumor injection as control.After administered 21 days, all mice were killed and the samples of tumor were removed.The state of gene Bcl-2 and P53 in samples was measured by immunology history biology method.Results:The positive rates of states of Bcl-2 are 46.1%(A), 58.3%(B) and 61.5% (C) respectively .There are significant differences between A and C. The positive rates of states of P53 are 38.4%(A), 41.67%(B) and 69.2%(C) respectively.There are also significant differences between A or B and C. Conclusion:DDP by intratumor injection may promote tumor apoptosis through regulating down Bcl-2 and P53 pathway,and these functions can be facilitated by a FFSG mixture.