温胃阳汤对胃动力低下大鼠胃电活动、胃窦肌间神经丛5-羟色胺能神经及其受体的影响

目的]观察温胃阳汤对胃动力低下大鼠胃电活动、胃窦肌间神经丛5-羟色胺（5-HT）能神经及其受体的影响.方法]将70只Wistar大鼠随机分为正常组（10只,雌雄各半）和模型组（60只,雌雄各半）,模型组每只大鼠按10 g/kg甘草煎剂灌胃,随机分为模型3d组、7d组、温胃阳汤低、中、高剂量组及吗丁啉组.温胃阳汤低、中、高各剂量组分别予浓度为0.743 g/ml、1.485 g/ml、2.970 g/ml的温胃阳汤灌胃,吗丁啉组予0.27 mg/ml吗丁啉混悬液（按100 g体重1 ml）灌胃.观察各组大鼠胃电活动的变化,并以免疫组化方法结合图像分析技术分析5-HT免疫反应阳性产物的分布、数量和免疫反应强度.以免疫印迹法分析大鼠胃窦组织中5-羟色胺受体（5-HTR）,计算出各组的蛋白相对表达量,以百分数表示各组的蛋白表达量.结果]一定剂量的甘草煎剂能明显抑制大鼠胃电活动,使大鼠窦组织5-HT免疫反应阳性神经纤维、神经节内阳性神经细胞体和平均光密度值明显减少,5-HTR的表达显著降低.而温胃阳汤低、中、高剂量组及吗丁啉组均可使胃动力低下大鼠胃窦组织5-HT免疫反应阳性神经纤维、神经节内阳性神经细胞体和带膨体的神经纤维增多,5-HTR的表达亦得到提高;其中中、高剂量组效果与吗丁啉组比较差异无统计学意义,与正常组比较差异有统计学意义（P＜0.05或P＜0.01）.结论]经温胃阳汤治疗后,胃窦肌间神经丛5-HT免疫反应阳性神经纤维和神经细胞体分布增多,5-HTR表达升高,5-HT能神经活性增强,表明温胃阳汤能通过5-HT能神经参与促胃动力作用.

The effect of Wenweiyang Decoction on the gastric electrical activity and 5-HT、 5-HTR in gastric antrum tissues of low gastric motility rats

Objective]To observe the effect of Wenweiyang Decoction（WD） on the gastric electrical activity and 5-HT,5-HTR in gastric antrum tissues of low gastric motility rats.Methods]Seventy Wistar rats were divided into normal group（10 rats）and model group（60 rats）at random.Each rat of model group was administered with liquorice decoction（10 g/kg）.After irritated stomach,model group was divided into model group of 3 days,model group of 7 days,low,moderate and high dosage groups of WD,and control group of domperidone.Control group was administered with domperidone,WD groups were administered with WD of different concentration：0.743 g/ml,1.485 g/ml and 2.970 g/ml respectively..Then the gastric electrical activity was tested and the distribution,amount,response intensity of 5-HT immune reaction product,5-HTR in in gastric antrum tissues were measured.Results] Liquorice decoction could establish rat model of low gastric motility,which showed obviously restricted the gastric electrical activity,lowered nerve fibers,nerve cell body and the average optical density of 5-HT immune reaction product,and lower 5 HTR in gastric antrum tissues..WD could improve the gastric electrical abnormalities,and increase 5-HT,5-HTR.The effect of medium and high dose groups of WD was similar to the domperidone group,all of the three groups showed statistically significant difference as compared with the normal group（P＜0.05 or P＜0.01）.Conclusion]WD could treat disease of gastrointestinal dismotility by increasing 5-HT and 5HTR in gastric antrum tissues of low gastric motility rats.