仁术健脾理气方对功能性消化不良大鼠胃排空功能及Ghrelin、5-HT、CGRP的影响

目的]研究仁术健脾理气方对碘乙酰胺（iodoacetamide,IA）诱导的功能性消化不良（FD）大鼠胃排空功能及胃促生长素（Ghrelin）、5-羟色胺（5-HT）、降钙素基因相关肽（CGRP）的影响,初步探讨仁术健脾理气方治疗FD的机制.方法]雄性10日龄SD幼鼠100只,随机分为正常组（30只）和造模组（70只）.采用0.1％ IA灌胃诱导大鼠FD模型.大鼠8周龄时进行模型评定,将造模成功的70只大鼠随机分为模型组（30只）、中药低剂量组（10只）、中药中剂量组（10只）、中药高剂量组（10只）和阳性药组（10只）5组.正常组与模型组予以0.9％氯化钠溶液1 ml/100 g灌胃,中药低、中、高剂量组分别给予生药含量0.477 5 g/ml、0.955 5 g/ml、1.911 g/ml的中药煎剂1 ml/100 g灌胃,阳性药组给予0.3 mg/ml多潘立酮溶液1 ml/100 g灌胃.采用苏木精-伊红染色观察大鼠胃组织病理变化.采用放射免疫法检测大鼠胃组织髓过氧化物酶（MPO）活性以及血清中Ghrelin、5-HT、CGRP的含量.结果]胃排空结果显示,中药高剂量组和阳性药组大鼠3h摄食量和胃排空率显著高于模型组（P＜0.05）,而3h后胃内食物残余量显著低于模型组（P＜0.05）.血清学结果显示,中药各剂量组和阳性药组FD大鼠血清中Ghre-lin和5-HT的含量均不同程度地升高（均P＜0.05）,且高剂量组与阳性药组比较差异无统计学意义;中药各剂量组和阳性药组FD大鼠血清中CGRP的含量均不同程度地降低（均P＜0.05）,且高剂量组与阳性药组比较差异无统计学意义.结论]仁术健脾理气方对IA诱导的FD大鼠具有加快胃排空、降低胃肠感觉过敏的作用,其机制可能与外周血 Ghrelin、5-HT含量升高、CGRP含量降低有关.

Effect of Renzhujianpiliqi formula on the gastric emptying and Ghrelin, 5-HT and CGRP in functional dyspepsia rats

Objective]To study the therapeutic effect of Renzhujianpiliqi formula （RF） on rats with functional dyspepsia（FD）,preliminary discuss its therapeutic mechanism.Methods] One-hundred tenday-old male rats were randomly divided into control group（n＝30）and model group（n=70）.0.1％ iodoacetamide（IA）was used to induce FD rats.Model rats were evaluated at the age of 8 weeks and the successful model of the 70 rats were randomly divided into model group（n＝30）,low-dose of Chinese herbal medicine（CHM）group（n=10）,moderate-dose of CHM group（n=10）,high-dose of CHM（n=10）and domperidone group（n=10）.The control group and the model group were administered with 0.9％ Sodium Chloride Solution,CHM groups were orally administered RF for 7 days.Hematoxylin eosin（HE） staining was applied to observe the pathological changes of gastric tissue of the rats.Radioimmunoassay was used to detect the activity of MPO in gastric tissue and Ghrelin,5-HT,CGRP conten.Results] Gastric emptying results showed：High-dose of CHM group and domperidone group rats consumed more solid food than the model group and presented a higher gastric emptying rate over 3-hour period（P＜0.05）,and the food in the stomach remnant was significantly lower than the model group after 3-hour period（P＜0.05）.CHM group and domperidone group increased the content of Ghrelin and 5-HT in serum of FD rats（bothP＜0.05） in different degrees,there was no significant difference between the high-dose of CHM group and domperidone group;CHM group and domperidone group decreased the content of CGRP in the serum of FD rats（P＜0.05） differently,there was no significant difference between the high-dose of CHM group and domperidone group.Conclusion]RF can promote the gastric motility and reduce gastrointestinal hypersensitivity in FD rats induced by IA,and its mechanism might be related to the increase of the Ghrelin and 5-HT content and the decrease of CGRP content.