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| 牛血小板衍化生长因子对人牙周膜成纤维细胞的生物学效应 | |
| 引用本文： | 陈哟 史俊南. 牛血小板衍化生长因子对人牙周膜成纤维细胞的生物学效应[J]. 牙体牙髓牙周病学杂志, 1998, 8(1): 25-28 |
| 作者姓名： | 陈哟 史俊南 |
| 作者单位： | 西安第四军医大学 |
| 摘 要： | 目的：观察在牛血小板衍化生长因子作用下人牙周膜成纤维细胞ＤＮＡ和胶原蛋白合成的情况。方法：采用体外细胞培养技术和核素掺入法。结果：２０ｎｇ／ｍｌ～６０ｎｇ／ｍｌ牛血小板衍化生长因子可明显促进人牙周膜成纤维细胞ＤＮＡ合成，４０ｎｇ／ｍｌ浓度使细胞ＤＮＡ合成在２４ｈ达最高峰；对细胞的胶原蛋白合成无明显促进作用。结论：牛血小板衍化生长因子对人牙周膜成纤维细胞ＤＮＡ合成有促进作用，在牙周组织的创伤修复中可能起重要作用。 |
| 关 键 词： | 血小板衍化生长因子 人牙周膜成纤维细胞 DNA合成 胶原蛋白合成 |
Biological effects of bovine platelet-derived growth factor on human periodontal ligament fibroblasts |
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| Chen Qiang,Shi Junnan,Ren Yushen et al School of Stomatology,The Fourth Military Medical University,Xi'an. Biological effects of bovine platelet-derived growth factor on human periodontal ligament fibroblasts[J]. Chinese Journal of Conservative Dentistry, 1998, 8(1): 25-28 | |
| Authors: | Chen Qiang Shi Junnan Ren Yushen et al School of Stomatology The Fourth Military Medical University Xi'an |
| Affiliation: | Chen Qiang,Shi Junnan,Ren Yushen et al School of Stomatology,The Fourth Military Medical University,Xi'an 710032 |
| Abstract: | Aim: DNA and collegen protein synthesis of human periodontal ligament fibroblast(HPLF) were determined by treating with bovine platelet-derived growth factor (bPDGF). Methods: 3H-TdR and 3H-proline incorporation in vitro. Results: bPDGF significantly accelerated DNA synthesis of HPLF in dose-dependent manner over a concertration range of 20ng/ml to 60ng/ml, with a maximal response at a concentration of 40ng/ml at 24h. bPDGF did not stimulate the collegen protein synthesis of HPLF. Conclusions: bPDGF could promote DNA synthesis of HPLF dependently, and may play an important role in wound healing of periodontal tissue. |
| Keywords: | platelet-derived growth factor human periodontal ligament fibroblast DNA synthesis collegen protein synthesis |
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