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| 白色念珠菌肠内定植导致局部调节因子表达增加 | |
| 引用本文： | 白晓东,刘贤华,肖光夏. 白色念珠菌肠内定植导致局部调节因子表达增加[J]. 中华医院感染学杂志, 2000, 10(3): 164-165 |
| 作者姓名： | 白晓东 刘贤华 肖光夏 |
| 作者单位： | 第三军医大学西南医院烧伤研究所!四川重庆400038(白晓东，肖光夏)，第三军医大学预防系复合伤研究所!四川重庆400038(刘贤华) |
| 基金项目： | 军队“九五”攻关项目“感染与免疫”资助 |
| 摘 要： | 目的 观察白色念珠菌肠腔内定植后局部调节功子表达变化。方法 采用无特殊病原菌小鼠定植白色念珠菌，分别于定植后３ｄ，７ｄ，１４ｄ活杀，观察如下指标：盲肠内白色念珠菌总数；潘氏结（ＰＰ）中斑点杂交观测ＩＬ－４，ＩＬ－６表达变化；通过原位杂交反映固有层中（ＬＰ）ＩＬ－６表达变化；ＥＬＩＳＡ测定肠匀浆中ＩＬ－６水平。结果 白色念珠菌总数以定植３ｄ，７ｄ较高，１４ｄ则明显下降；ＰＰ中ＩＬ－４表达以定植１４ｄ |
| 关 键 词： | 细胞因子 白色念株菌 粘膜免疫 |
Colonization of Candida albicans in Intestine Inducing Increased Expression of Cytokine in Gut Associated Lymph Tissue |
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| BAI Xiao dong ,LIU Xianhua ,XIAO Guangxia. Colonization of Candida albicans in Intestine Inducing Increased Expression of Cytokine in Gut Associated Lymph Tissue[J]. Chinese Journal of Nosocomiology, 2000, 10(3): 164-165 | |
| Authors: | BAI Xiao dong LIU Xianhua XIAO Guangxia |
| Affiliation: | BAI Xiao dong 1,LIU Xianhua 2,XIAO Guangxia 1 |
| Abstract: | OBJECTIVE To investigate the change in IL-4 and IL-6 expression in local intestinal mucosal immunity tissue during colonization of Candida albicans.METHODS The total number of C.albicans in intestine was calculated by culture;IL- expression in PP was observed by spot hybridization,meanwhile IL-6 expression in LP was observed by in situ hybridization. RESULTS The changes in total number of Candida were obvious after inoculation.At PCD 3 and PCD 7 ,the number of candidas was higher,but it decreased at PCD 14 .At PCD 14 ,IL-4 expression in PP was the highest, while at PCD 7 IL-6 expression in LP was the top one.CONCLUSIONS Local increasing in IL-4 and IL-6 expression may be induced by colonization of C.albicans. |
| Keywords: | Cytokine Candida albicans Mucosal immunity |
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