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| 川木通与其混伪品和近缘种的ITS2条形码分子鉴定 | |
| 引用本文： | 刘美子,李美妮,姚辉,刘萍. 川木通与其混伪品和近缘种的ITS2条形码分子鉴定[J]. 环球中医药, 2011, 4(6): 446-450. DOI: 10.3969/j.issn.1674-1749.2011.06.012 |
| 作者姓名： | 刘美子 李美妮 姚辉 刘萍 |
| 作者单位： | 1. 100193,中草药物质基础与资源利用教育部重点实验室,中国医学科学院,北京协和医学院药用植物研究所;中国人民解放军总医院药品保障中心中药房 2. 中国医学科学院,北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室,100193 3. 中国人民解放军总医院药品保障中心中药房 |
| 基金项目： | 国家自然科学基金(81073001) |
| 摘 要： | 目的 分析川木通及其混伪品和近缘种的ITS2条形码序列,探讨川木通及其混伪品和近缘种鉴定的新方法.方法 对样品进行DNA提取,PCR扩增ITS2片段并进行双向测序,CodonCode Aligner拼接序列,MEGA5分析川木通的种内遗传距离及与混伪品和近缘物种的种间遗传距离,预测ITS2二级结构,应用BLAST方法进... |
| 关 键 词： | 川木通 ITS2序列 分子鉴定 混伪品 DNA条形码 |
Molecular identification of Clematidis Armandii Caulis and its adulterants and closely related species by ITS |
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| LIU Mei-zi,LI Mei-ni,YAO Hui,et al.. Molecular identification of Clematidis Armandii Caulis and its adulterants and closely related species by ITS[J]. Global Chinese Medicine, 2011, 4(6): 446-450. DOI: 10.3969/j.issn.1674-1749.2011.06.012 | |
| Authors: | LIU Mei-zi LI Mei-ni YAO Hui et al. |
| Affiliation: | LIU Mei-zi,LI Mei-ni,YAO Hui,et al.The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China |
| Abstract: | Objective To identify Clematidis Armandii Caulis and its adulterants and closely related species using ITS2 barcode,and study the feasibility of this new method.Methods ITS2 regions were amplified by PCR technology and sequenced bidirectionally.Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner.The Interspecific and intraspecific genentic distances of Clematidis Armandii Akebiae and its adulterants and closely related species were computed by MEGA5.Predict the ITS... |
| Keywords: | Clematidis Armandii Caulis ITS2 Molecular identification Adulterants DNA barcoding |
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