丁苯酞预处理对大鼠局灶性脑缺血再灌注损伤的干预研究

目的探讨预防性应用丁苯酞对SD大鼠脑缺血再灌注损伤是否具有保护作用及其可能机制。方法 36只SD大鼠随机分为假手术组、对照组、预处理组(均n=12);预处理组制模前给予丁苯酞80 mg/(kg.d)灌胃14 d。改良Zea Longa线栓法制备大鼠右侧大脑中动脉阻断(MCAO)局灶性脑缺血再灌注模型,于再灌注后24 h行Zea Longa评分标准进行神经功能缺损评分;2%氯化三苯基四氮唑(TTC)染色测量梗死体积;免疫组化法观察Caspase-3的表达。结果对照组和预处理组神经功能缺损评分较假手术组均明显增高(P均<0.01);预处理组神经功能缺损评分较对照组明显降低(P<0.01);对照组和预处理组脑梗死体积较假手术组均明显增高(P均<0.01);预处理组脑梗死体积较对照组明显减少(P<0.01);对照组和预处理组的Caspase-3表达较假手术组均明显增高(P均<0.01);预处理组的Caspase-3表达较对照组明显降低(P<0.01)。结论预防性应用丁苯酞可以减轻SD大鼠神经功能缺损症状,缩小梗死体积,减少Caspase-3表达;预防性应用丁苯酞具有神经保护作用,其机制可能与减少Caspase-3表达抑制细胞凋亡有关。

Intervention study on injury of focal cerebral ischemia reperfusion in rats pretreated with Butylphthalide

Objective It is to approach whether Butylphthalide pretreated can relieve the injury of focal cerebral ischemia reperfusion in Sprague-Dawley(SD) rats and the possible mechanism.Methods 36 healthy male SD rats were randomly divided into sham operated group,control group and pretreated group in which there were 12 rats.Pretreated group was given Butylphthalide 80 mg/(kg·d) gavage for 14 days before ischemia reperfusion.The right middle cerebral artery occlusion(MCAO) focal ischemia reperfusion rat models were established with the improved Longa-Zea method.Neurologic impairment score was evaluated with Zea-Longa’s standards at 24 h after ischemia reperfusion.The volume of cerebral infarction was measured with 2% triphenyl tetrazolium chloride(TTC) staining.The expression of Caspase-3 was observed with immunohistochemistry method.Results Neurologic impairment scores in control group and pretreated group were obviously higher than that in sham operated group(both P<0.01).Neurologic impairment score in pretreated group was obviously lower than that in control group(P<0.01).The volumes of cerebral infarction in control group and pretreated group were obviously higher than that in sham operated group(both P<0.01).The volume of pretreated group was obviously lower than that in control group(P<0.01).The expressions of Caspase-3 in control group and pretreated group were obviously higher than that in sham operated group(both P<0.01).The expression of Caspase-3 in pretreated group was obviously lower than that in control group(P<0.01).Conclusion Butylphthalide pretreated can relieve the symptoms of neurologic impairment score in SD rats,reduce the volume of cerebral infarction and decrease the expression of Caspase-3.Butylphthalide pretreated has neuroprotective effect and the mechanism may be related to apoptosis inhibition by reducing the expression of Caspase-3.