脑出血大鼠脑内移植神经干细胞后再生相关因子的表达

目的观察大鼠脑出血模型脑内移植神经干细胞(NSCs)后脑源性神经营养因子(BDNF)和血管内皮生长因子(VEGF)的表达及对血管生成的影响。方法 SD大鼠140只,随机分为假手术组(A组)、脑出血模型组(B组)、NSCs培养液移植组(C组),NSCs移植组(D组),每组35只。免疫组织化学方法观察各组术后6 h、1、4、7、14、21和28天BDNF、VEGF及CD34标记的微血管密度的变化,并进行相关分析。结果与A组比较,B组、C组BDNF的表达1天达高峰(P<0.05),D组表达高峰延迟至第4天,至28天仍高于A组(P<0.05);D组在所有时间点与B组比较,差异有统计学意义(P<0.05);D组在4天后的5个时间点与C组比较,差异有统计学意义(P<0.05)。B组、C组VEGF和微血管密度表达模式相同,均在1天达高峰;D组呈双高峰表达,在第4天和14天,至28天仍高于B组、C组的表达水平(P<0.05);相关分析显示,VEGF与微血管密度呈正相关(r=0.911,P<0.05)。结论脑出血大鼠脑内移植NSCs后,脑内BDNF及VEGF维持长时间的高表达,微血管密度增加。

Expression of regeneration-related factor in rats with intracerebral hemorrhage after transplantation of neural stem cells

Objective To observe the expression of BDNF and VEGF and vasculogenesis in rats with intracerebral hemorrhage(ICH) after transplantation of NSCs. Method 140 SD rats were di-vided randomly into sham operated group(group A), model group(group B), NSCs culture medi-um transplanted group(group C) and NSCs transplanted group(group D). NSCs were transplan-ted into the subventricular zone in rat models of ICH,The expression changes of BDNF,VEGF,microvessel density(MVD) at 6 h and on days 1,4,7,14,21 and 28 after ICH was observed by means of immunohistochemical technique. Results Compared with group A, the expression of BDNF peaked on first day in groups B and C,and the expression peak was delayed to 4th day in group D,but maintained higher expression than group A on 28th day (P<0.05). At all time points,positive cell counts between group D and group B had significant difference (P<0.05).On days 4,7,14,21 and 28,positive cell counts between group D and group C had significant difference (P < 0.05). The expression levels of VEGF and MVD peaked on first day in groups B and C,and were higher in group C than in group B on days 1,4. Group D had double expression peaks on 4th day and 14th day,and maintained higher expression than in groups B,C on 28th day (P < 0.05). VEGF showed positive correlation with MVD (r=0.911 ,P<0.05). Conclusions The high expression of BDNF and VEGF is maintained for a long time and MVD increases in rats with cerebral hemorrhage after transplantation of NSCs.