人参皂甙对不同代龄人胚肺成纤维细胞的增殖及Cyclin D1基因表达的影响

目的 :探讨人参皂甙对衰老的人胚肺成纤维细胞增殖及CyclinD1基因表达的调节作用 ,以进一步研究人参皂甙抗细胞衰老的机制。方法 :用体外培养的人胚肺成纤维细胞为细胞衰老模型 ,通过细胞流式分析、免疫组化及RT PCR方法 ,观察了人参皂甙对成纤维细胞的增殖周期、DNA合成 ,CyclinD1mRNA和蛋白质表达情况。结果 :衰老的成纤维细胞用人参皂甙连续处理 4代后 ,处于G1期的细胞数明显减少 ,进入S期的细胞由 16 7%增加到 33% (P <0 0 5 ) ;CyclinD1mR NA表达比对照组下降 ,CyclinD1蛋白质表达由原来的 77 1± 2 9明显下降为 5 2± 6 3(P <0 0 0 1) ,DNA合成增加 17% (P <0 0 5 )。人参皂甙对低代龄细胞的CyclinD1RNA合成有所增加 ,对CyclinD1蛋白质、DNA的作用影响不大。结论 :人参皂甙对人胚肺成纤维细胞具有双向作用 ,对高代龄细胞具有促进细胞增殖 ,调节CyclinD1基因表达的作用

Effect of Ginsensides on Proliferation and Cyclin D1 Gene Expression in Human Fibroblasts

Objective:In order to investigate the antiaging mechnaism of Ginsensides,the effect of Ginsensides on proliferation and Cyclin D1 expression in senescent human fibroblasts were analyzed.Methods:Normal human diploid embryonic lung fibroblasts were used as a senescent model to observe the effect of Ginsensides on cell cycle,DNA synthesize and Cyclin D1 gene expression by FACS,immunohisto chemistry and RT PCR.Results:After treating with Ginsensides,the number of G1 phase of aging human fibroblasts decreased and S phase fibroblasts increased from 16.7% to 33%( P<0 005 ),the level of Cyclin D1 mRNA was lower than contral,Cyclin D1 protein decreased 25% too,and DNA synthesize level increased 17%( P<0 05 ).Ginsensides play a less role in increasing Cyclin D1 mRNA level and regulating cell cycle in younger fibroblasts.Conclusion:Ginsensides have bi directional effect on fibroblasts.In contrast to in yougner fibroblasts,it can accelerate cell proliferation and regulate Cyclin D1 expression in senescent human fibroblasts.