口腔白斑及鳞状细胞癌中c-Myc及hTERT蛋白表达的研究

目的 研究c-Myc和hTERT蛋白在口腔白斑和鳞状细胞癌中的表达及其与二者发生发展的关系。方法采用免疫组化方法检测口腔白斑48例、鳞状细胞癌4l例和lO例正常口腔粘膜组织中c-Mye和hTERT蛋白的表达，并进行统计学处理分析。结果在正常口腔粘膜组、单纯增生性白斑组、异常增生性白斑组和OSCC组c-Myc蛋白表达的阳性率分别为0（0／10）、21．43％（3／14）、52．94％（18134）、75．61％（31141），组间差异有统计学意义（P〈0．05）；在正常口腔粘膜组、单纯增生性白斑组、异常增生性白斑组和OSCC组hTERT蛋白表达的阳性率分别为0（0／10）、7．14％（1／14）、32．36％（11／34）、58．54％（24／41），组间差异有统计学意义（P〈0．05）。结论c-Myc蛋白、hTERT蛋白的表达与细胞增殖及肿瘤的发生密切相关，可能是检测OLK恶变潜能的指标。

Study on the Expression of c-Myc and hTERT in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Objective To investigate the expression of c-Myc and hTERT and their relation between the development of OLK and OSCC. Methods Immunohistochemical staining technique was used to detect expression of c-Myc and hTERT protein in 48 cases of OLK,41 cases of OSCC and 10 cases of normal oral mucosa.All data were analyzed statistically. Results The expression rates of c-Myc protein in normal control group,simple hyperplasia OLK,dysplasia OLK and OSCC were 0(0/10),21.43%(3/14),52.94%(18/34) and 75.61%(31/41) respectively,showed a significant difference among them(P<0.05);the expression rates of hTERT protein in normal control group,simple hyperplasia OLK,dysplasia OLK and OSCC were 0(0/10),7.14%(1/14),32.36%(11/34) and 58.54%(24/41) respectively,showed significant difference among them(P<0.05). Conclusion The expression of c-Myc and hTERT protein may be closely related to cell proliferation,differentiation and carcinogenesis.They may be useful index for evaluating the malignant potential of OLK.