p16基因对脑胶质瘤细胞生长及放射敏感性的影响

目的探索p16基因在脑胶质瘤发生发展过程中的作用及p16基因与肿瘤细胞放射敏感性间的关系.方法将外源野生型p16基因导入胶质瘤细胞株U251、C6,筛选阳性克隆.同时以空载体质粒为对照,免疫组化检测p16基因表达,用MTT法测定细胞生长曲线及肿瘤杀伤率.结果转染p16基因的U251、C6细胞有外源p16基因的整合及表达,克隆形成率减少,生长速度明显减慢,对辐射的敏感性增强.结论导入外源野生型p16基因可抑制胶质瘤细胞恶性增殖,提高胶质瘤细胞对辐射的敏感性.

Effects of p16 gene on the growth and Radiation Sensitivity of Human Glioma Cell Line

Aim: To determine the effects on cell growth and radiation sensitivity of p16 gene in human glioma cell line. Methods:p16 gene was transfected into U251 and C6 glioma cell lines by lipofectin. Integration and expression of exogenous p16 gene were detected. MTT colorimetric assay was used to measure cell growth and cytotoxity of radiation. Results: Ex- pression of p16 gene was demonstrated by immunohistochemistry. The growth rate of both p16 gene transfected U251 and C6 reduced. Both Wt p16 positive U251 and C6 were more sensitive to radiation than control cell lines. Conclusion: The expres- sion of p16 gene can inhibit the growth of glioma and increase radiation sensitivity of human glioma.