雷公藤红素对血管平滑肌细胞内游离Ca2+和H+浓度的影响

目的；观察雷公藤红素对血管平滑肌细胞内游离Ｃａ＾２＋和Ｈ＾＋浓度的影响。方法：采用荧光染色法，参激光扫描焦显微镜动态研究了不同浓度的胎牛血清对单个血管平滑肌内游离Ｃａ＾２＋和Ｈ＾＋浓度变化的影响效应。结果：３０％胎牛血清可以引起细胞内一快速的，持续的游离Ｃａ＾２＋浓度升高，而３０％胎牛血清加０．３ｍｇ／Ｌ雷公藤红素则可以产生抑制细胞内游离Ｃａ＾２＋浓度升高的效应；

Effect of Tripterine on Concentration of Free Calcium and Hydrogen in Vascular Smooth Muscle Cells

To investigate the effects of tripterine on concentration of free calcium (Ca 2 ) and hydrogen(H ) in vascular smooth muscle cells (VSMC). Methods: By using fluorescence staining method, changes of the free calcium and hydrogen in single VSMC were studied with laser confocal scanning analyzer after different concentrations of fetal calf serum (FCS), tripterine and FCS plus tripterine were added into the culture pools respectively. Results: The concentration of intracellular Ca 2 increased rapidly, sequentially and significantly after addition of 30% FCS (P<0 05), 0.3mg/L tripterine plus 30% FCS had inhibition effect on the concentration of the intracellular Ca 2 , 20% FCS reduced the concentration of intracellular H significantly (P<0 05), and 0.2mg/L tripterine caused a significant increasing concentration of H (P<0 05). Conclusion: Tripterine has an antagonistic action on the increasing concentration of intracellular Ca 2 induced by FCS, the inhibitory effect of tripterine on the Ca 2 channel of VSMC membrane may be responsible for this new mechanism.