| 高效液相色谱法测定鼻窦炎口服液中栀子苷含量 |
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| 引用本文： | 陈刚.高效液相色谱法测定鼻窦炎口服液中栀子苷含量[J].中国药业,2011,20(12):30-31. |
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| 作者姓名： | 陈刚 |
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| 作者单位： | 湖南省张家界市药品检验所,湖南张家界,427000 |
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| 摘 要： | 目的建立鼻窦炎口服液中栀子苷的含量测定方法。方法采用高效液相色谱(HPLC)法。以岛津Shimpack VP-ODS C18柱(250 mm×4.6 mm,5μm)为色谱柱,以乙腈-0.2%磷酸(12∶88)为流动相,柱温25℃,流速1.0 mL/min,检测波长为238 nm。结果栀子苷进样量在0.235～1.767μg范围内与峰面积有良好的线性关系,r=0.999 9(n=6);平均加样回收率为99.11%,RSD=0.67%(n=6)。结论该方法灵敏、简便、结果准确、重现性好,可用于鼻窦炎口服液的质量控制。
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| 关 键 词： | 栀子苷 鼻窦炎口服液 高效液相色谱法 |
Determination of Geniposide in Nasosinusitis Oral Solution by HPLC |
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| Chen Gang.Determination of Geniposide in Nasosinusitis Oral Solution by HPLC[J].China Pharmaceuticals,2011,20(12):30-31. |
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| Authors: | Chen Gang |
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| Institution: | Chen Gang (Zhangjiajie Municipal Institute for Drug Control,Zhangjiajie,Hunan,China 427000) |
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| Abstract: | Objective To establish a HPLC method for geniposide contents in Nasosinusitis Oral Solution.Methods The HPLC method was adopted and the Shimpack VP-ODS C18 column（250 mm×4.6 mm,5 μm）was used at 25 ℃.The mobile phase was acetonitrile-0.2%phosphoric acid（12 ∶88） at the flow rate of 1.0 mL/min.The detection wavelength was at 238 nm.Results The linear range of calibration curve was 0.235-1.767 μg for geniposide（r=0.999 9）,the average recovery rate was 99.11%,RSD=0.67%（n=6）.Conclusion The methods is highly sensitive quick and accurate with good repeatability,which can be used for the quality control of Nasosinusitis Oral Solution. |
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| Keywords: | geniposide nasosinusitis oral solution HPLC |
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