不对称PCR-SSCP检测鼻咽癌p130基因研究

目的:探讨p130基因改变与鼻咽癌发生、发展的关系。方法:提取鼻咽癌组织DNA,用不对称单链构象多态性(SSCP)PCR技术,分别对20例散发性鼻咽癌患者及3个鼻咽癌高发家系进行性p130基因第11、17和19外显子纯合性缺失及突变研究,以20例慢性鼻咽炎患者的鼻咽部组织做为对照。结果:散发性鼻咽癌患者有相同的p130 E11、E17纯合性缺失2例,未发现p130E19缺失;对照组中检出1例p130E11、E17纯合性缺失,也无p130E19缺失;鼻咽癌家系未发现缺失和突变。结论:鼻咽癌中p130 E11、E17纯合性缺失可能涉及到p130/Rb2基因的改变,在鼻咽癌的发生发展中起一定的作用,而且p130/Rb2基因作为一种抑癌基因可能成为鼻咽癌新的基因治疗手段的候选基因。

Research of p130 gene in nasopharyngeal carcinoma detecting by asymmetry PCR-SSCP

Objective:To research the correlation between p130 gene variation and onset of nasopharyngeal carcinoma.Methods: After extracting the DNA of nasopharyngeal carcinoma tissues,detecting loss of homozygosity and mutation of p130 gene exon 11,exon 17 and exon 19 in 20 sporadic nasopharyngeal carcinoma patients and 3 ancestries,with 20 nasopharyngeal tissues of chronic nasopharyngitis patients as control group.Results: Two sporadic nasopharyngeal carcinoma patients had loss of homozygosity of exon 11 and exon 17 and no loss of homozygosity of exon 19;one chronic nasopharyngitis patient had loss of homozygosity of exon 11 and exon 17,There was no loss of homozygosity and mutation found in 3 ancestries and are not loss of homozygosity and mutation of exon 19 in all cases.Conclusion: Detecting loss of homozygosity of p130 gene exon 11 and 17 in nasopharyngeal carcinoma may be related to the variation of p130/Rb2 gene,and plays some role in the onset and developing of nasopharyngeal carcinoma;As an antioncogene,p130/Rb2 gene is likely to become the candidate gene of genetherapy in the new therapy methods of nasopharyngeal carcinoma.