代谢性酸中毒对大鼠红细胞碳酸酐酶活性的影响

目的：探讨代谢性酸中毒对大鼠红细胞碳酸酐酶（CA）活性的影响。方法：60只Wistar大鼠随机分为实验组和对照组，实验组采用0．28mol／LNH4Cl喂饲，构建大鼠代谢性酸中毒模型，对照组予0．9％NaCl喂饲。分别在24、48和72h采用量电法测定两组大鼠红细胞CA活性。结果：实验组大鼠24h红细胞CA活性较对照组明显增高（P〈0．05）；但随着代谢性酸中毒的加重，48h和72h实验组大鼠红细胞CA活性被抑制，明显低于对照组（P〈0．05）。结论：代谢性酸中毒显著影响大鼠红细胞碳酸酐酶活性。

Effect of metabolic acidosis on carbonic anhydrase activity in rat erythrocytes

Objective:To investigate the effect of metabolic acidosis on carbonic anhydrase(CA) activity in rat erythrocytes.Methods: Metabolic acidosis model was induced by addition of 0.28 mol/L NH_4Cl to drinking water for 24 h,48 h and 72 h in Wistar rats(n=10).Carbonic anhydrase activity of erythrocytes was assayed by electrometry method.Results: The activity of carbonic anhydrase was significantlyincreased in the metabolic acidosis group(AG) compared with the control group(CG)(P<0.05).But with the decrease of pH,this effect was disappeared.Conclusions: Metabolic acidosis plays a very important role in change of CA activity during the early stage of metabolic acidosis.