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| 苦叶七种质资源的随机扩增多态性DNA分析 | |
| 引用本文： | 樊冬丽,牛西午,马小军,肖小河,鄢丹,廖庆文.苦叶七种质资源的随机扩增多态性DNA分析[J].时珍国医国药,2006,17(12):2471-2473. |
| 作者姓名： | 樊冬丽 牛西午 马小军 肖小河 鄢丹 廖庆文 |
| 作者单位： | 1. 中国医学科学院,中国协和医科大学,药用植物研究所,北京,100094 2. 山西省农业科学院,山西,太原,030006 3. 中国人民解放军第302医院中医药研究所,北京,100039 |
| 摘 要： | 目的探讨不同苦叶七资源在分子水平上的遗传多样性。方法采用随机扩增多态性DNA(RAPD)技术对30个苦叶七地方品种进行检测,并用NTSYS软件对扩增结果进行统计和聚类。结果80个随机引物中有10个引物的扩增产物具有多态性,每个多态性引物平均可扩增出6个片段。聚类分析表明,全部材料可划分为五个类群。结论苦叶七种质资源在分子水平上存在明显的遗传差异。 |
| 关 键 词： | 苦叶七 随机扩增多态性DNA 遗传多样性 |
| 文章编号： | 1008-0805（2006）12-2471-03 |
| 收稿时间： | 2006-04-13 |
| 修稿时间： | 2006-09-16 |
Random Amplified Analysis of Fagopyrum tataricum from Different Germplasm Resources |
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| FAN Dong-li,NIU Xi-wu,MA Xiao-jun,XIAO Xiao-he,YAN Dan,LIAO Qing-wen.Random Amplified Analysis of Fagopyrum tataricum from Different Germplasm Resources[J].Lishizhen Medicine and Materia Medica Research,2006,17(12):2471-2473. | |
| Authors: | FAN Dong-li NIU Xi-wu MA Xiao-jun XIAO Xiao-he YAN Dan LIAO Qing-wen |
| Institution: | 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China; 2. Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China; 3. Institute of Chinese Medicine ,302 Hospital of PLA ,Beijing 100039, China |
| Abstract: | Objective To explore the genetic diversity of Fagopyrum tataricum on molecular level.Methods Random amplified polymorphic DNA(RAPD)was used to test 30 Fagopyrum tataricum.The NTSYS software was used to analyze the marks.Results Among 80 arbitrary primers,10 were selected to amplify polymorphic produces,6 polymorphic bands were amplified by each polymorphic primer on the average.Using UPGMA method,all the tested accessions could be clustered into five groups.Conclusion There actually existed much genetic diversity on molecular level among Fagopyrum tataricum germplasm resources. |
| Keywords: | Fagopyrum tataricum RAPD Genetic diversity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |