MCL1蛋白在胃癌中的表达及临床意义

目的：MCL1（myeloid cell leukemia-1）与多种恶性肿瘤的发生、发展及转移相关。本文从临床样本和细胞水平检测胃癌组织中MCL1蛋白的表达水平,探讨MCL1蛋白在胃癌发生、发展中的作用。方法：采用S-P免疫组化法检测正常胃黏膜、癌旁胃黏膜和胃癌组织芯片中MCL1蛋白的表达,Western blot检测MCL1蛋白在人胃癌BGC823、SGC7901、MKN28细胞中的表达。结果：免疫组化结果显示,MCL1蛋白表达定位于细胞的胞浆,着色呈黄色至棕黄色;正常胃黏膜、癌旁胃黏膜、胃癌中MCL1蛋白阳性表达率分别为18.18%（4/22）、54.17%（26/48）、83.33%（75/90）;MCL1蛋白在胃癌中的表达高于癌旁胃黏膜及正常胃粘膜,癌旁胃黏膜与正常胃粘膜MCL1蛋白表达亦具有明显差异（P〈0.05）;MCL1蛋白阳性表达率在伴淋巴结转移组为93.48%（43/46）,无淋巴结转移组为72.72%（32/44）,两者差异有显著性意义（P〈0.05）;而与性别、年龄和肿瘤大小无关。S-P免疫组化法与Western blot结果显示MCL1蛋白表达与胃癌分化程度呈负相关。结论：MCL1蛋白表达与胃癌发生、分化程度和淋巴结转移相关。

Expression of MCL1 Protein in Gastric Carcinoma and Its Clinical Significance

Objective:To investigate the expression of MCL1 protein in gastric carcinoma and to explore the relation-ship between MCL1 protein expression and the development of gastric carcinoma.Methods:Expression of MCL1 protein was examined immunohistochemically by the streptavidin-peroxidase conjugated method(SP) in normal mucosa,paracancerous tissues and gastric carcinoma.Western blot was used to detect the expres-sion of MCL1 protein in BGC823,SGC7901 and MKN28 gastric cancer cell lines.Results:MCL1 protein ex-pre...