On the synergy between silver nanoparticles and doxycycline towards the inhibition of Staphylococcus aureus growth |
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| Authors: | Heloiza F. O. Silva,Rayane P. de Lima,Fernanda S. L. da Costa,Edgar P. Moraes,Maria C. N. Melo,Celso Sant’ Anna,Mateus Eugê nio,Luiz H. S. Gasparotto |
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| Affiliation: | Biological Chemistry and Chemometrics Research Group, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal 59072-970 RN Brasil, +55 84 33422323 ; Laboratory of Medical Bacteriology, Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59078-970 RN Brasil ; Laboratory of Biotechnology – Labio, National Institute of Metrology, Quality and Technology – Inmetro, Duque de Caxias 25250-020 RJ Brazil |
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| Abstract: | In a previous paper (RSC Adv., 2015, 5, 66886–66893), we showed that the combination of silver nanoparticles (NanoAg) with doxycycline (DO) culminated in an increased bactericidal activity towards E. coli. Herein we further investigated the metabolic changes that occurred on Staphylococcus aureus upon exposure to NanoAg with the help of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) coupled with multivariate data analysis. It has been discovered that the combination of DO with NanoAg produced metabolic changes in S. aureus that were not simply the overlap of the treatments with DO and NanoAg separately. Our results suggest that DO and NanoAg act synergistically to impede protein synthesis by the bacteria.Silver nanoparticles conjugated with doxycycline act synergistically to halt S. aureus growth via inhibition of protein synthesis. |
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