| 中药材蜈蚣及其混伪品DNA条形码鉴别研究 |
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| 引用本文： | 张红印,陈俊,贾静,刘冬,石林春,张辉,宋经元,姚辉.中药材蜈蚣及其混伪品DNA条形码鉴别研究[J].中国中药杂志,2014,39(12):2208-2211. |
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| 作者姓名： | 张红印 陈俊 贾静 刘冬 石林春 张辉 宋经元 姚辉 |
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| 作者单位： | 中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;长春中医药大学, 吉林 长春 130117;福建农林大学, 福建 福州 350002;长春中医药大学, 吉林 长春 130117;长春中医药大学, 吉林 长春 130117;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;长春中医药大学, 吉林 长春 130117;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193 |
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| 基金项目： | 北京协和医学院创新团队发展计划项目 |
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| 摘 要： | 利用DNA条形码技术对蜈蚣药材进行鉴别，为蜈蚣药材鉴定提供新的方法。该研究以COI条形码序列为基础，对蜈蚣实验样品进行DNA提取，PCR扩增和双向测序，用MEGA6.0软件对所有5个物种的50份样品进行序列比对和邻接（NJ）树构建。结果显示实验样品均可以获得COI序列，蜈蚣药材与其混伪品COI序列种间平均K2P距离为0.222，种间最小K2P 距离为0.190，基于COI序列构建的邻接（NJ）树中少棘巨蜈蚣单独聚在一枝，与其混伪品可以相互区分。因此基于COI条形码序列可以准确鉴定蜈蚣及其混伪品。
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| 关 键 词： | 蜈蚣 DNA条形码 COI 鉴定 |
| 收稿时间： | 2014/3/14 0:00:00 |
Identification of Scolopendra subspinipes mutilans and its adulterants using DNA barcode |
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| ZHANG Hong-yin,CHEN Jun,JIA Jing,LIU Dong,SHI Lin-chun,ZHANG Hui,SONG Jing-yuan and YAO Hui.Identification of Scolopendra subspinipes mutilans and its adulterants using DNA barcode[J].China Journal of Chinese Materia Medica,2014,39(12):2208-2211. |
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| Authors: | ZHANG Hong-yin CHEN Jun JIA Jing LIU Dong SHI Lin-chun ZHANG Hui SONG Jing-yuan and YAO Hui |
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| Institution: | Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Changchun University of Chinese Medicine, Changchun 130117, China;Fujian Agriculture and Forestry University, Fuzhou 350002, China;Changchun University of Chinese Medicine, Changchun 130117, China;Changchun University of Chinese Medicine, Changchun 130117, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Changchun University of Chinese Medicine, Changchun 130117, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China |
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| Abstract: | In this study, the COI barcode was used to identify the Scolopendra medicinal materials and its adulterants in order to provide a new method for the identification of Scolopendra. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence alignment and NJ tree construction was carried out by MEGA6.0 software. The results showed that the COI sequences can be obtained from all experimental samples. The average inter-specific K2P distance of Scolopendra was 0.222 and the minimum inter-specific distance was 0.190. All the Scolopendra subspinipes mutilans medicinal samples clustered into a clade in the NJ tree and can be distinguished from its adulterants. In a conclusion, COI can be used to correctly identify Scolopendra medicinal materials, and it will be a potential DNA barcode for identifying other animal medicinal materials. |
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| Keywords: | Scolopendra subspinipes mutilans DNA barcoding COI identification |
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