| 基于ITS2序列鉴定中药材升麻及其混伪品 |
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| 引用本文： | 任伟超,马孝熙,于俊林,马伟,孙伟. 基于ITS2序列鉴定中药材升麻及其混伪品[J]. 中国中药杂志, 2014, 39(12): 2184-2188 |
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| 作者姓名： | 任伟超 马孝熙 于俊林 马伟 孙伟 |
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| 作者单位： | 中国中医科学院 中药研究所, 北京 100700;黑龙江中医药大学 药学院, 黑龙江 哈尔滨 150040;中国中医科学院 中药研究所, 北京 100700;通化师范学院 制药与食品科学学院, 吉林 通化 134000;黑龙江中医药大学 药学院, 黑龙江 哈尔滨 150040;中国中医科学院 中药研究所, 北京 100700 |
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| 基金项目： | 国家“重大新药创制”科技重大专项（2013ZX09301307，Z131100006513017）；教育部长江学者和创新团队发展计划项目（IRT1150） |
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| 摘 要： | 该研究选用ITS2序列对升麻及其混伪品进行分子鉴定，从而确保用药安全。实验采用DNA条形码技术，对升麻及其混伪品的ITS2核基因片段进行扩增并双向测序，经CodonCode Aligner V3.7.1拼接后，利用MEGA 5.0软件对相关数据分析，构建邻接（NJ）系统聚类树进行鉴定分析。结果表明升麻药材的3个基原物种：大三叶升麻Cimicifuga heracleifolia、升麻C. foetida、兴安升麻C. dahurica，其ITS2序列长度分别为217，219，219 bp，3个基原物种ITS2序列与混伪品的种间平均K2P距离大于其种内平均K2P距离，ITS2系统发育树显示，升麻的3个基原物种均各自聚为一单系分支，并与其他混伪品明显分开。ITS2序列能够有效准确地鉴别中药材升麻及其混伪品。
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| 关 键 词： | DNA条形码 ITS2 升麻 鉴定 |
| 收稿时间： | 2014-03-17 |
Identification of Cimicifugae Rhizoma and its adulterants using ITS2 sequence |
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| REN Wei-chao,MA Xiao-xi,YU Jun-lin,MA Wei and SUN Wei. Identification of Cimicifugae Rhizoma and its adulterants using ITS2 sequence[J]. China Journal of Chinese Materia Medica, 2014, 39(12): 2184-2188 |
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| Authors: | REN Wei-chao MA Xiao-xi YU Jun-lin MA Wei SUN Wei |
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| Affiliation: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Pharmacy College, Heilongjiang University of Chinese Medicine, Harbin 150040, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;College of pharmaceutical and Food Science, Tonghua Normal University, Tonghua 134000, China;Pharmacy College, Heilongjiang University of Chinese Medicine, Harbin 150040, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | In order to identify Cimicifugae Rhizoma from its adulterants and to ensure its safe use,the internal transcribed spacer 2 (ITS2) sequence of Cimicifugae Rhizoma and its adulterants were amplified and bidirectionally sequenced by DNA barcoding technology. Sequence assembly and consensus sequence generation were performed by the CodonCode Aligner V3.7.1. The genetic distances were computed by MEGA 5.0. Identification analyses were performed using neighbor-joining (NJ) methods. The length of ITS2 sequence of the three origin plants of Cimicifugae Rhizoma include Cimicifuga heracleifolia, C. foetida, C. dahurica was 217, 219 and 219 bp, respectively. Their intraspecific genetic distance was much lower than the interspecific genetic distance with their closely related species. The NJ tree of ITS2 indicated that the three origin plants of Cimicifugae Rhizoma formed a monophyletic clade, Cimicifugae Rhizoma and its adulterants could be distinguished clearly. The authors proposed that ITS2 sequence was suitable for the authentication of Cimicifugae Rhizoma and its adulterants. |
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| Keywords: | DNA barcoding ITS2 Cimicifugae Rhizoma identification |
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