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HPLC法测定不同采收时期杜仲叶中绿原酸的含量
引用本文:茹建永,乔孝伟. HPLC法测定不同采收时期杜仲叶中绿原酸的含量[J]. 中国药房, 2008, 19(27): 2112-2114
作者姓名:茹建永  乔孝伟
作者单位:河南义马煤业集团公司总医院,义马市,472300
摘    要:
目的:优选杜仲叶的最佳采收时期。方法:以高效液相色谱法测定不同采收时期杜仲叶中绿原酸的含量:色谱柱为Kro-masil-C18(250mm×4.6mm,5.0μm),流动相为乙腈-0.4%磷酸溶液(11:87),检测波长为327nm。结果:杜仲叶中绿原酸的含量在7月份时最高,为3.178%。结论:7月份是杜仲叶的最佳采收时期。

关 键 词:杜仲叶  绿原酸  高效液相色谱法  含量  采收时期

Determination of Chorogenic Acid in Eucommia ulmoides at Different Harvest Time by HPLC
RU Jian-yong,QIAO Xiao-wei. Determination of Chorogenic Acid in Eucommia ulmoides at Different Harvest Time by HPLC[J]. China Pharmacy, 2008, 19(27): 2112-2114
Authors:RU Jian-yong  QIAO Xiao-wei
Affiliation:(General Hospital of Henan Yima Coal Company, Yima 472300, China)
Abstract:
OBJECTIVE: To optimize the harvest time of Eucommia ulmoides .METHODS: The content of Chlorogenic acid in Eucommia ulmoides harvested at different time was determined by HPLC on Kromasil-C18(250mm×4.6mm,5.0μm) .The mobile phase consisted of acetonitrile- 0.4% HaPO4 solution( 11 : 87) with a detection wavelength of 327 nm. RESULTS: The content of Chlorogenic acid in Eucommia ulmoides harvested during July was the highest(at 3.178% ).CONCLUSION: July turned out to be the optimum harvest time for Eucommia ulmoides.
Keywords:Eucommia ulmoides  Chlorogenic acid  HPLC  Content  Harvest time
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