ECHO30所致无菌性脑膜炎暴发的病原学检测分析

目的]明确引起无菌性脑膜炎暴发的病原体，从病原学角度探讨引起暴发的原因。方法]按照WHO规定的标准方法进行病毒的分离、型别鉴定以及血清中和抗体试验；采用传统的技术测定分离株的生物学特性。结果]43份大便标本共分离19株肠道病毒阳性株，阳性率44.12％，型别鉴定均为ECHO30；分离株具备强毒株的“D”特征；76.92％双份血清抗体呈4倍升高。结论]2003年济南章丘市无菌性脑膜炎暴发的病原体为具备强毒株特征的ECHO30。

Analysis of Etiological Inspection on the Outbreak of Aseptic Meningitis Caused by Echovirus Type 30 (ECHO30)

Objective]To identify pathogen linked with the outbreak of aseptic meningitis,and to probe the reason for the outbreak on etiology.Methods]Virus isolation,serotype identification and neutralization test were used according to the WHO standard methods.The biological characteristics of isolates were identified by conventional method.Results]19 strains of human enterovirus with 44.12% positive rate were isolated from 43 stool specimens.All isolates were identified to single echovirus30(ECHO30).The ECHO30 isolates had "D" feature with enhanced virulence.The serological diagnosis also showed that ECHO30 was the pathogen of the outbreak because 76.92% of double sera collected from acute-phase and convalescent-phase had 4 times rising for neutralization antibody against ECHO30.Conclusion]The outbreak of aseptic meningitis was caused by a single ECHO30 with enhanced virulence in Zhangqiu,Ji'nan of China in 2003.