食品中单核细胞增生李斯特氏菌快速检测方法研究

[目的 ]　建立一种快速准确检验食品中单核细胞增生李斯特氏菌的方法。　 [方法 ]　以miniVIDAS仪结合API系统 ,将该法与传统方法进行比较。　 [结果 ]　该方法能检出 <10个 /mL模拟样品中的单核细胞增生李斯特氏菌 ,与食品中常见的细菌无交叉反应 ,能对非单核细胞增生李斯特氏菌作出正确鉴定。在 4天内能作完全鉴定结果。对 2 2 6份实样的检测 ,检出率为 11.5 %。　 [结论 ]　以miniVIDAS仪结合API系统建立的方法具有特异性好、灵敏度高、快速简便的特点 ,是一种较好的检测单核细胞增生李斯特氏菌的方法

A study of fast examination of Listeria monocytogenes in foods

To establish a fast and nicety method for examine the listeria monocytogenes(LMO) in foods. Combining miniVIDAS with API-System to set up a new method and compared the result of new method with that of the routine method. The sensitivity of the new method is <10cfu/mL listeria monocytogenes in simulative food, in the meantime,there were no crossed-reactions with usual bacterium in foods.The new method could identify the non-LMO correctly.This method could give final report in four days.This method had been used to examine 226 actual samples with positive rate of LMO 11.5%. [Conclusion] The miniVIDAS-API method has following features:fast,high sensitivity and high specificity,and is a good method to test the LMO in foods.