两种心气虚证动物模型的对比研究

目的比较非疾病状态下生理性功能失调和中医病证结合两种心气虚动物模型的中医证候特点。方法采用两种造模方式,即大鼠水环境站台睡眠剥夺法致生理性功能失调和结扎大鼠冠状动脉前降支主干致心梗后心衰病证结合动物模型,观察大鼠一般状况、心率、呼吸频率、力竭性游泳时间、血流动力学等指标,应用临床心气虚证的诊断标准,对两种动物模型进行诊断。结果睡眠剥夺大鼠精神亢奋、反抗剧烈、心率加快、呼吸频率增加、力竭性游泳时间缩短和心功能亢进;心梗后心衰大鼠精神萎靡、少动、心率加快、呼吸频率增加、力竭性游泳时间缩短和心功能减退。结论睡眠剥夺法所致大鼠模型是对机体施加功能性造模因素所致,该模型具有证候表现复杂、变化不稳定的特点;它不同于心梗心衰病证结合模型局部器质性病变导致的有稳定规律的证候表现。

Comparison of two types of animal models with heart-qi insufficiency syndrome

Objective To compare the syndrome characteristics of two types of animal models with heart-qi insufficiency syndrome combined physical function disorder and syndromes of Chinese medicine under the non-ill condition. Methods The animal models were established by using two methods,the model of physical function disorder(PFD) was established by sleeping deprivation in rats on water stand,and the model of postinfarction heart failure(PHF) was established by ligation of the front descending coronary artery.The general state,heart rate,respiratory frequency,exhausted swimming time and hemodynamic index were observed.The two kinds of animal models were didgnosed by the clinical diagnostic standard of syndrome of heart-qi insufficiency.Results The rats of PFD model had stimulated spirit,(acute) antagonism,high heart rate and respiratory rate frequency,shortened exhausted swimming time and (hyperfunction) of heart.The rats of PHF model had listless spirit,less movement,high heart rate and(respiratory) rate frequency,shortened exhausted swimming time and hypofunction of heart.Conclusion The rat model of PFD established by functional factors has the characteristics of complicated syndrome(manifestations) and unstable changes.It differs from the model of PHF which has stable syndrome(manifestations) induced by local unself-healing organic disease.