HSP70在缺血再灌注大鼠肾脏中的表达及意义

目的观察大鼠肾脏缺血及缺血再灌注(I/R)诱导热休克蛋白70(HSP70)的表达及意义。方法制作大鼠肾脏缺血及I/R模型,缺血组于缺血5、15和30min,I/R组于再灌注2、5、8、24、48、72h不同时间摘取肾脏,采用免疫组化方法检测肾脏HSP70的表达,同时采用HE染色,观察肾脏病理改变。结果在缺血组各时间点肾脏HSP70的表达无明显差异(P>0·05);肾脏I/R后,随时间延长,HSP70表达明显增强(P<0·05);肾脏病理显示,缺血时肾组织呈现局灶性及弥散性血管周围水肿,I/R后随时间延长肾脏损伤加重,损伤部位主要在肾小管,表现为空泡样变、小管萎缩、上皮细胞脱落及肾小球囊腔扩张等。结论HSP70在I/R后表达增强,但肾脏仍损伤严重,提示HSP70在肾脏I/R过程中没有起到主要的保护作用。

Significance of HSP70 expression in rat kidneys after ischemia-reperfusion

Objective To explore the significance of Heat-Shock Protein70 (HSP70) expression in ischemia-reperfusion(I/R)injury of rat kidneys.Methods Ischemia model and ischemia-reperfusion model of rat kidney were established.The kidneys at different time points were chosen for study.HSP70 expression in kidney tissues was determined by immunohistochemical method.Pathologically,kidneys injury was examined after HE staining.Results In ischemia group,HSP70 expression had no obvious difference from that of control group (P>0.05).In I/R group,HSP70 levels increased significantly from 2h to 72h after I/R injury (P<0.05).According to pathological examination,ischemia group showed focal and diffuse edema around the blood vessels in the kidneys and the I/R group presented more severe kidney injury (renal tubules were most affected,presenting vacuolar degeneration,tubular atrophy,epithelial exfoliation and dilated glomerular capsule).Conclusion HSP70 expression in rat kidneys increased in the I/R group,but the injury of kidneys was still very severe,indicating that HSP70 played a minor protective role in the kidney I/R process.