还原型谷胱甘肽对凝血功能的影响

本研究探讨还原型谷胱甘肽（GSH）对凝血功能的影响。随机留取正常凝血血浆标本及其混合血浆标本，加入常规剂量及各浓度梯度GSH，采用凝固法检测加药物前后血浆凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、纤维蛋白原（FIB）及凝血酶时间（TT），统计分析加药前后PT、APTT、FIB及TT检测结果的差异，并对其检测结果与GSH的浓度作线性回归分析；随机抽取常规剂量给药21例患者，采集给药前后血液标本，统计分析给药前后血浆PT、APTT、FIB及TT检测结果的差异。结果表明，体外试验中，加入常规剂量（500mg／L）GSH后血浆FIB、APTT、PT及TT检测结果与加药前检测结果之M的差异有统计学意义（P〈0．01），且当加入的GSH浓度分别达2．5、2．5、10、1280mg／L时，FIB、APTT、PT及TT、的测定结果与加药前之间的差异具有统计学意义（P〈0．01），且4项测定结果与血浆中加入的GSH浓度存在线性关系；21例试验患者用药后血浆FIB、APTT、PT及TT检测值与用药前有显著性差异（P〈0．001），其中共19例FIB、20例FT、20例TF和21例APTT检测结果高于用药前。结论：在体内外实验中，GSH可以影响凝血检测结果。

Effect of Glutathione on Blood Coagulation Function

The pourpose of this study was to investigate the effect of glutathione （GSH） on blood coagulation. The normal plasma samples and mixed plasma samples were taken randomly, and into which the normal dose and different concentration of GSH were added. The prothrombin time （PT）, activated partial thromboplastine time （AFvr）, fibrinogen（FIB） and thrombin time（TT） were detected by using coagulation method before and after treatment with GSH. The detection results of normal plasma and mixed plasma containing GSH of different concentration were compared and analyzed with linear regression. The results showed that the APTT and FIB values of the plasma containing 2.5 mg/L glutathione or more, PT values of the plasma containing 10 mg/L glutathione or more, and TT values of the plasma containing 1250 mg/L glutathione or more were significantly different from those results of normal plasma or mixed plasma（ P 〈 0.01 ）. There was a linear relation between all of the detection results of PT, APTT, FIB, TT and glutathione concentrations. The results of TT, AFTT, PT and FIB detection in patient plasma were statistically different （P 〈0.01 ） before and after treatment with normal concentration GSH. It is concluded that glutathione can influence detection results of coagulation function.