交链孢酚单甲醚对人胚食管上皮的作用

用人胚食管上皮体外培养,经交链孢酚单甲醚(Alternariol monomethyl ether,AME)处理后,食管上皮底层细胞比对照组的层次明显增多,细胞排列紧密紊乱,细胞核浆比增加,核不规则,大小不等.浓染,可见核分裂相,且有乳头状向内增生。该变化与甲基硝基亚硝基胍(MNNG)和甲基苯基亚硝胺(MPNA)所诱发的人胚食管上皮癌前病变相类似。结果提示,AME与人的食管癌发生很可能有关。

The effect of alternariol monomethyl ether(AME)on the cultured esophageal epithelium of the human fetal

Alternaria altemata was isolated from the grains in the areas of high incidence ofesophageal, cancer. The alternariol monomethyl ether (AME), the metabolite ofAlternaria alternata, had the mutagenic activity. There is no report about themutagenic effect of AME on the epithelial tissue of human esophagus. The aimsof this experiment are to detect the effect of AME on the epithelial tissue of theesophagus of human fetal and to find out the relation between AME and the ca-use of esophageal cancer. The cultured esophageal epithelial tissue of human fetalwere exposed to AME and continued to be cultured for one week. Then the eso-phageal epithelial tissue were detected with pathological methods.The results showedthat in the experimental groups, the basal calls of the esophageal epithelium werehyperplastic, disorderly arranged and with papillary growth, and the layers of thebasal calls increased. The nucleum of cells were big and were deeply stained. Nu-clear divisional phases were found. The changes in the experimental groups wereobviously different from those in the control group. The same changes were obser-ved in the positive groups which were treated with MNNG and Methyl-phenly-nitrosamine. It is suggested that AME may possibly be one of the causes of humanesophageal cancer.