| 原核表达、体外折叠的HLA-G5促进人外周血单核细胞产生TNF |
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| 引用本文： | 褚利霞,吴雄文,夏芳珍,鈡茂华,李青,翁秀芳,卢小玲,张彩娥,陆盛军,梁智辉,龚非力.原核表达、体外折叠的HLA-G5促进人外周血单核细胞产生TNF[J].现代免疫学,2006,26(3):222-225. |
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| 作者姓名： | 褚利霞 吴雄文 夏芳珍 鈡茂华 李青 翁秀芳 卢小玲 张彩娥 陆盛军 梁智辉 龚非力 |
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| 作者单位： | 华中科技大学同济医学院免疫学系,武汉430030 |
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| 摘 要： | 通过原核表达获得HLA-G5重链和轻链(β2m),经初步纯化后,利用稀释法与人工合成的九肽(KGPPAALTL)一起进行体外复性折叠,形成HLA-G5-抗原肽复合物,经非变性PAGE/Western blot和夹心ELISA法鉴定,证实复性后成功获得天然构象的HLA-G5。利用PBMC受LPS刺激后产生TNF的特点,观察原核表达、体外折叠的HLA-G5对人PBMC分泌TNF的影响。TNF分泌量采用TNF敏感的L929细胞进行生物法测定,结果显示原核表达、体外折叠的HLA-G5促进人PBMC分泌TNF-α。
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| 关 键 词： | HLA-G5-抗原肽复合物 稀释复性 TNF |
| 文章编号： | 1001-2478（2006）03-0222-04 |
| 收稿时间： | 2005-09-23 |
| 修稿时间： | 2006-02-24 |
The in vitro refolded prokaryotic HLA-G5 promotes the TNF production in human peripheral blood mononuclear cells |
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| CHU Li-xia,WU Xiong-wen,XIA Fang-zhen,ZHONG Mao-hua,LI Qing,WENG Xiu-fang,LU Xiao-ling,ZHANG Cai-e,LU Sheng-jun,LIANG Zhi-hui,GONG Fei-li.The in vitro refolded prokaryotic HLA-G5 promotes the TNF production in human peripheral blood mononuclear cells[J].Current Immunology,2006,26(3):222-225. |
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| Authors: | CHU Li-xia WU Xiong-wen XIA Fang-zhen ZHONG Mao-hua LI Qing WENG Xiu-fang LU Xiao-ling ZHANG Cai-e LU Sheng-jun LIANG Zhi-hui GONG Fei-li |
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| Institution: | Department of Immunolagy Tongji Medical College. Huazhong University of Science and Technology. Wuhan 430030, China |
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| Abstract: | In this study,the highly-expressed and primarily-purified prokaryotic HLA-G5 heavy chain(HC),and the HLA class I light chain(LC,ie.β_2m),refolded in vitro in the presence of a synthetic antigenic peptide(KGPPAALTL),to form the HLA-G5 antigenic peptide complex.The conformation of the refolded complex was detected by HLA class I specific mAb W6/32 with native-PAGE/Western blot and ELISA,the effect of the refolded HLA-G5 on the TNF production of human(PBMC) was observed with bioassay by using TNF-sensitive L929 cell line.The results showed the prokaryotic HLA-G5-antigenic peptide complex was successfully produced,sharing the epitope of HLA class I,and the complex was able to promote the TNF production of human PBMC in vitro. |
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| Keywords: | HLA-G5-peptide complex dilution TNF |
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