Tumor interstitial fluid pressure may regulate angiogenic factors in osteosarcoma |
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| Authors: | Saminathan S. Nathan Andrew G. Huvos Jorge E. Casas‐Ganem Rui Yang Irina Linkov Rebecca Sowers Gene R. DiResta Richard Gorlick John H. Healey |
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| Affiliation: | 1. Musculoskeletal Oncology Service, Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, 5, Lower Kent Ridge Road, Singapore 119074, Singapore;2. Department of Pathology, Orthopaedic Surgery Service, Memorial Sloan‐Kettering Cancer Center, New York 10021, New York (affiliated with the Weill Medical College of Cornell University);3. Department of Surgery, Memorial Sloan‐Kettering Cancer Center, New York 10021, New York (affiliated with the Weill Medical College of Cornell University);4. Section of Hematology and Oncology, Department of Pediatrics, The Children's Hospital at Montefiore, 3415 Bainbridge Avenue, New York 10467 (affiliated with Albert Einstein College of Medicine) |
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| Abstract: | We have previously shown that osteosarcomas (OS) have states of increased interstitial fluid pressure (IFP), which correlate with increased proliferation and chemosensitivity. In this study, we hypothesized that constitutively raised IFP in OS regulates angiogenesis. Sixteen patients with the clinical diagnosis of OS underwent blood flow and IFP readings by the wick‐in‐needle method at the time and location of open biopsy. Vascularity was determined by capillary density in the biopsy specimens. We performed digital image analysis of immunohistochemical staining for CD31, VEGF‐A, VEGF‐C, and TPA on paraffin‐embedded tissue blocks of the biopsy samples. Clinical results were validated in a pressurized cell culture system. Interstitial fluid pressures in the tumors (mean 33.5 ± SD 17.2 mmHg) were significantly higher (p = 0.00001) than that in normal tissue (2.9 ± 5.7 mmHg). Pressure readings were significantly higher in low vascularity tumors compared to high vascularity tumors (p < 0.001). In the OS cell lines, growth in a pressurized environment was associated with VEGF‐A downregulation, VEGF‐C upregulation, and TPA upregulation. The reverse was seen in the OB cell line. Growth in the HUVEC cell line was not significantly inhibited in a pressurized environment. Immunohistochemical assessment for VEGF‐A (p = 0.01), VEGF‐C (p = 0.008), and TPA (p = 0.0001) translation were consistent with the findings on PCR. Our data suggests that some molecules in angiogenesis are regulated by changes in IFP. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:1520–1525, 2008 |
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| Keywords: | interstitial fluid pressure osteosarcoma angiogenesis lymphangiogenesis |
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