首页 | 本学科首页   官方微博 | 高级检索  
     


Depressed natural killer cell activity due to decreased natural killer cell population in a vitamin E-deficient patient with Shwachman syndrome: reversible natural killer cell abnormality by α-tocopherol supplementation
Authors:N. Adachi  M. Migita  T. Ohta  A. Higashi  I. Matsuda
Affiliation:(1) Department of Paediatrics, Kumamoto University School of Medicine, Honjo 1-1‐1, Kumamoto City, Kumamoto 860, Japan Tel.: 096‐344‐5191; Fax: 096‐366‐3471, JP
Abstract:Natural Killer (NK) cell activity was examined in a 16-month-old Japanese boy with Shwachman syndrome associated with severe vitamin E deficiency. As evaluated by 51Cr-release assay from K562 cells, NK cell activity was constantly decreased. After 8 weeks of oral α-tocopherol (α-Toc) supplementation (100 mg/day), NK cell activity had normalised. When α-Toc supplementation was interrupted for 16 weeks, NK cell activity again decreased. Flow cytometry of peripheral lymphocytes revealed a lowered number of CD16+ CD 56 fraction, which has the most potent NK cell activity. Single cell-in-agarose assay, to investigate the binding and cytolytic activity of NK cell at the single cell level, revealed that the number of NK cells which bind to K562 cell was decreased, but that the cytolytic activity of the individual binding cell was relatively unaffected. A second supplementation of α-Toc for 8 weeks successfully restored NK cell activity, the number of cells expressing NK cell markers and the number of K562-binding cells as compared to the age-matched normal range. Conclusion These results indicate that severe vitamin E deficiency caused impaired NK cell activity due to a decrease in the number of CD16+ CD56 NK cells and that this abnormality is reversible with α-Toc supple‐mentation. Received: 30 January 1996 and in revised form: 19 November 1996 / Accepted: 22 November 1996
Keywords:Vitamin E deficiency       Natural killer cell activity       Shwachman syndrome α  -Tocopherol supplementation
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号