蛋白激酶Cε在前列腺癌组织中表达的临床意义

目的 探讨蛋白激酶C ε(PKCε)在不同病理类型前列腺组织中的表达及与前列腺癌病理分级、分期的关系。 方法 正常前列腺(NP)组织标本10例、前列腺增生(BPH)组织标本10例、癌旁(PC)组织标本10例、前列腺癌(PCa)组织标本43例。免疫组化法检测各组织中PKCε的表达情况，分析PKCε表达与不同病理类型及PCa分级、分期的关系。 结果 PCa组PKCε表达阳性27例，BPH组无阳性表达，NP组1例，PC组2例，差异有统计学意义(P＜0.05)。PCa组Gleason评分≥8分组中PKCε表达阳性12/13例，2～4分组4/10例，5～7分组11/20例，组间差异有统计学意义(P＜0.05)。T3期PKCε表达阳性10/12例，T4期9/10例，T1、T2期分别为1/6例和7/15例，高分期与低分期组PKCε表达差异有统计学意义(P＜0.05)。PCa转移组PKCε表达阳性9/10例，未转移组18/33例，组间差异有统计学意义(P＜0.05)。PCa患者血清PSA≤20 ng/ml者PKCε表达阳性7/15例，＞20 ng/ml组20/18例，组间差异无统计学意义(P＞0.05)。 结论 PCa组织中PKCε的表达率较高，并且与PCa病理分级、分期呈正相关，临床上可考虑作为PCa预后因子之一。

Expression and clinical significance of novel protein kinase C ε in prostate cancer tissuss

Objective To explore the expression of novel protein kinase C ε (PKCε) in normal prostate (NP) tissue, benign prostate hyperplasia(BPH), peficancerous (PC) tissue and prostate cancer (Pca), and study its correlation with the grade and stage of Pca.MethodsTen NP slides, ten BPH slides, ten PC slides and 43 Pca slides were collected from our hospital. These slides were routinely proceased and analyzed according to the requirement of immunohistochemical staining. Tumors were classified according to the 2002 TNM staging system. The grading system used in the study was based on the Gleason grade.Results We was found that the expression of PKCεs in Pca (27/43) were significantly higher than those in NP(1/10), BPH (0/10) and PC (2/10) tissue, and the difference was statistically significant ( P ＜0.05 ). With regard to grade of prostate cancer, the expression of PKCε in Pca with Gleason score ≥8 group (12/13) was higher than the Gleason score 2 -4 group (4/10) and the Gleason score 5 -7 group (11/20). The difference was statistically significant (P ＜ 0.05 ). Moreover, the T3 and T4 stages had a more positive rate (10/12 & 9/10) than the T1 and T2 stages( 1/6 &7/15). There is statistically significant difference between early and advanced stage of prostate cancer ( P ＜ 0. 05 ). Furthermore, the positive expression of PKCε in prostatic carcinoma samples increased significantly in the metastasis group (9/10)compared to the non-metastasis group ( 18/33 ) ( P ＜ 0. 05 ), but the difference was not statistically significant between the concentration of prostate-specific antigen in blood serum ( P ＞ 0. 05 ).Conclusions PKCε is expressed in prostate cancer, and it correlates with the grade and stage of prostate cancer. PKCε may be related to the origin and the development of Pca, and it may be used as a prognostic factor for Pca.