In vitro bactericidal effect of Nd:YAG laser on Actinomyces israelii |
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Authors: | Paolo Vescovi Stefania Conti Elisabetta Merigo Tecla Ciociola Luciano Polonelli Maddalena Manfredi Marco Meleti Carlo Fornaini Jean-Paul Rocca S amir Nammour |
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Institution: | 1. Faculté de Médécine et Chirurgie de Liège, Liege, Belgium 3. Unit of Oral Medicine, Oral Pathology and Laser-assisted Oral Surgery, Department of ENT/Dental/Ophthalmological and Cervico-Facial Sciences, University of Parma, Parma, Italy 2. Section of Microbiology, Department of Pathology and Laboratory Medicine, University of Parma, Parma, Italy 4. Sezione di Odontostomatologia, Azienda Ospedaliero-Universitaria di Parma, Via A. Gramsci 14, 43100, Parma, Italy 5. Teleo Laboratory, Faculty of Dentistry, University of Nice Sophia Antipolis, Nice, France
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Abstract: | A bactericidal effect has been reported by the use of near-infrared laser light on both Gram-positive and Gram-negative bacteria. The aim of this study was to evaluate the effect of Nd:YAG laser on Actinomyces israelii, filamentous bacteria causing cervicofacial actinomycosis. Experiments were realized on bacterial cells in saline suspension or streaked on Mueller–Hinton (MH) agar plates with or without India ink. Laser application was performed in Eppendorf tubes with different powers and frequencies for 40 s; bacterial suspensions were then streaked on agar plates and incubated at 35 °C in proper conditions for 5 days before colony enumeration. A reduction of colony number variable from 60.13 to 100 % for powers of 2, 4, and 6 W at 25–50 Hz of frequency was observed in comparison with growth control. For agar plates, laser application was performed with different powers at 50 Hz for 60 s. A growth inhibition was observed after 5 days of incubation on MH plates with powers of 6 W and on MH–ink plates with all applied powers. This preliminary study showed a bactericidal effect caused by Nd:YAG laser application worthy to be evaluated in further experiments in vivo. |
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