| 异源双链分析和单链构象多态性分析在基因突变检测中的作用 |
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| 引用本文： | 刘晓军,郭辰虹,邹雅群,卢勇,陈丙玺,龚瑶琴. 异源双链分析和单链构象多态性分析在基因突变检测中的作用[J]. 山东大学学报(医学版), 2001, 39(2): 166-167 |
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| 作者姓名： | 刘晓军 郭辰虹 邹雅群 卢勇 陈丙玺 龚瑶琴 |
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| 作者单位： | 山东大学医学院医学遗传学教研室;山东大学医学院医学遗传学教研室;山东大学医学院医学遗传学教研室;山东大学医学院医学遗传学教研室;山东大学医学院医学遗传学教研室;山东大学医学院医学遗传学教研室 |
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| 摘 要： | 评价异源双链分析(HET)和单链构象多态性分析(SSCP)在基因突变检测中的作用。方法同时应用两种方法检测23例肺腺癌标本中p53基因和p16基因突变情况。结果用HET法检出6例p53基因突变和5例p16基因突变,用SSCP法检出3例p53基因突变和1例p16基因突变,联合应用HET和SSCP方法检出7例p53基因突变和5例p16基因突变。结论检测基因突变HET优于SSCP,联合应用HET和SSCP能提高基因突变检出率.
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| 关 键 词： | 异源双链分析 单链构象多态性分析 基因p53 基因p16 DNA突变分析 |
| 文章编号： | 1000-0496(2001)02-0166-02 |
| 修稿时间： | 2000-04-27 |
COMPARISON OF HETERODUPLEXANALYSIS AND SSCP IN GENE MUTATION SCREENING |
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| LIU Xiao jun,GUO Chen hong,ZOU Ya qun,et al. COMPARISON OF HETERODUPLEXANALYSIS AND SSCP IN GENE MUTATION SCREENING[J]. Journal of Shandong University:Health Sciences, 2001, 39(2): 166-167 |
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| Authors: | LIU Xiao jun GUO Chen hong ZOU Ya qun et al |
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| Abstract: | Objective:To assess the roles of single stranded conformation polymorphism(SSCP)and heteroduplex polymorphism(HET)in screening gene mutation.Methods:The mutations of p53 gene and p16 gene in tumor tissues from 23 patients with lung cancer were analyzed by SSCP and HET.Results:Six mutatons in p53 gene and 5 mutations in p16 gene were detected by HET technique.Three mutations in p53 gene and 1 mutation in p16 gene were detected by SSCP technique.Seven mutations in p53 gene and 5 mutations p16 gene were detected by HET and SSCP together.Conclusion:HET is better than SSCP in screening gene mutation.Detection rate can be improved by HET and SSCP together. |
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| Keywords: | Heteroduplex analysis Single stranded conformation polymorphism analysis p53 gene p16 gene DNA mutational analysis |
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