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| 低浓度紫杉醇抑制SPCA-1细胞增殖的实验研究 | |
| 引用本文： | 李妍,任旷,马爱新,鞠晓红,李强,李文彬. 低浓度紫杉醇抑制SPCA-1细胞增殖的实验研究[J]. 吉林医药学院学报, 2009, 30(2) |
| 作者姓名： | 李妍 任旷 马爱新 鞠晓红 李强 李文彬 |
| 作者单位： | 吉林医药学院基础医学院,吉林,吉林,132013 |
| 基金项目： | 吉林省科技厅资助课题 |
| 摘 要： | 目的研究低浓度紫杉醇(PTX)对非小细胞肺癌SPCA-1细胞增殖、细胞周期和细胞凋亡的影响。方法用不同浓度PTX处理SPCA-1细胞16 h,去除含药培养液继续培养56 h,计数细胞并计算细胞生长抑制率。用0、1.5、3和6 nmol PTX,以及PTX与10μmol/L SP600125联合处理细胞16 h,流式细胞术分析细胞周期和细胞凋亡。结果低浓度PTX可显著抑制SPCA-1细胞增殖,其IC50、IC70和IC90值分别为1.75、3.25和4.50 nmol/L。0、1.5、3和6 nmol/L的PTX分别诱导(0.5±0.36)%、(17.0±0.55)%、(27.0±0.63)%和(21.0±3.02)%细胞发生凋亡,而10μmol/L SP600125使低浓度PTX诱导的细胞凋亡显著下降。低浓度PTX也具有抑制有丝分裂的作用,0、1.5、3和6 nmol/L的PTX分别导致(22.7±1.68)%、(19.4±1.43)%、(26.2±0.97)%和(42.6±3.36)%细胞阻滞于G2/M期。结论低浓度PTX可通过诱导细胞凋亡和有丝分裂阻滞抑制SPCA-1细胞生长,而JNK激酶抑制剂可下调PT... |
| 关 键 词： | 紫杉醇 非小细胞肺癌 凋亡 |
Experimental study of inhabitation of lower concentration of paclitaxel to the proliferation of SPCA-1 cel |
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| LI Yan,REN Kuang,MA Ai-xin,JU Xiao-hong,LI Qiang,LI Wen-bin. Experimental study of inhabitation of lower concentration of paclitaxel to the proliferation of SPCA-1 cel[J]. Journal of Jilin Medical College, 2009, 30(2) | |
| Authors: | LI Yan REN Kuang MA Ai-xin JU Xiao-hong LI Qiang LI Wen-bin |
| Affiliation: | Department of Etiology;Jilin Medical College;Jilin City;Jilin Province;132013;China |
| Abstract: | Objective To study the effect of lower concentration paclitaxel on the proliferation,cell cycle,apoptosis and mitosis of non-small cell SPCA-1.Methods SPCA-1 cells were treated by different concentration of paclitaxel for 16 hours,paclitaxel containing medium was replaced by drug free medium.After additional 56 hours,cells were counted and the growth inhibition rates were calculated.SPCA-1 cells were treated by 0,1.5,3 and 6 nmol/L paclitaxel with or without 10 μmol/L SP600125 for 16 hours,the cell cycle an... |
| Keywords: | paclitaxel NSCLC apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |