自制枸橼酸钠干粉抗凝管在EDTA依赖性假性血小板减少症中的应用研究

目的:制备最佳抗凝浓度的枸橼酸钠干粉抗凝管,使EDTA依赖性假性血小板减少症患者的血小板计数更加简便、准确。方法:1配置不同浓度枸橼酸钠干粉抗凝管,优选其最佳抗凝浓度、抗凝血量及检测时间范围。2使用不同抗凝管同时采集4种不同类型患者静脉血,进行血小板计数,对EDTA依赖性假性血小板减少症患者同时进行末稍血手工法血小板计数及抗凝血涂片染色观察血小板聚集情况。结果:1枸橼酸钠干粉抗凝管的最佳抗凝浓度为12.8mg/管,在抗凝血量200~800μl、上机检测时间10~120min的条件下计数血小板可得到较准确结果。2最佳抗凝浓度的枸橼酸钠干粉抗凝管在门诊体检者、血小板增多患者、真性血小板减少症患者中,血小板结果与EDTA-K2真空抗凝管血小板结果比较,差异无统计学意义;在EDTA依赖性假性血小板减少症患者中,血小板结果与末稍血手工法计数血小板结果比较,差异亦无统计学意义,枸橼酸钠干粉抗凝血涂片染色观察血小板呈单个散在分布。结论:自制的枸橼酸钠干粉抗凝管可简便、准确地纠正EDTA依赖性假性血小板减少症患者的血小板数量。

Study on application of self-made sodium citrate anticoagulant tube for EDTA-dependent Pseudo-thrombocytopenia

Objective： To prepare the optimal concentration of Sodium Citrate anticoagulant tube for platelet count of EDTA-dependent Pseudo-thrombocytopenia. Method：①Sodium Citrate anticoagulant tubes with different concentration were prepared, and then the anticoagulant concentration, blood volume and detection time range were optimized. ②Venous blood were collected from four different types of patients with different anticoagulant tubes, platelet count were detected using peripheral blood and platelet aggregation was observed with anticoagulant blood smear for EDTA-PTCP. Result： ①The results of platelet count were more accurate at the optimal anticoagu- lant concentration of 12.8 mg, blood volume with the range of 200μl to 800 μl, and the detection time under 10 to 120 min.②There was no significant difference between Sodium Citrate anticoagulant tube （251.53 ±68.35） and EDTA-K2 vacuum anticoagulation tube （257.02± 72.28） for healthy subjects, thrombocytosis patients, true thrombocytopenia patients and EDTA-PTCP patients. Meanwhile no statistical difference was existed between EDTA-PTCP patients with the result of platelet count （193.89±50.62） and peripheral blood （199.14±47.99）. And platelet was scattered using Sodium Citrate anticoagulant blood smear. Conclusion：It would be simple and re- liable to correct platelet reduction affected by EDTA-PTCP with self-made sodium citrate anticoagulant tube.