山羊颈椎间盘纤维环组织成骨潜能的体内观察

目的：观察山羊颈椎椎间融合器内填充松质骨、纤维环组织后在体内的组织学变化过程，以了解纤维环组织在骨融合过程中的成骨潜能。方法：实验山羊按常规颈椎前路减压、内同定术式施术，术中随机取C2～C6椎间隙中相邻的两个间隙．每个间隙各置入两枚钛合金颈椎窄心螺纹式柱状内同定器(CHTF)，分别填充单纯松质骨(A组)：松质骨 纤维环(B组)：纤维环(C组)及空白对照(D组)。术后应用X线片、颈椎CT扫描等影像学检查及组织切片观察植骨融合及局部组织反应情况。结果：X线片及CT示内置CHTF与椎体的骨一金属界面周围有骨组织生长．CHTF与椎体终板接触部位有成骨现象，骨桥形成。A组切片观察示新生软骨、骨小梁存在，原植入骨坏死：B组纤维组织有坏死．原骨小梁、纤维环周围新牛骨存在，新生软骨堆积；C组术后6周纤维组织内有纤维软骨存在．术后12周新生软骨存在；D组术后6周组织学观察无阳性染色结果，术后12周有少量新生软骨。结论：颈椎间盘纤维环组织有成骨潜能，成骨形式可能是成纤维细胞的软骨化骨。

In vivo study on osteogenic potential of annulus fibrosus in goat cervical intervertebral disc

Objective:To explore the osteogenic potential annulus fibrosus of cervical intervertebral disc in vivo in goat model.Method:Cervical hollow threaded fixator(CHTF) filled with different combinations of cancellous bone and annulus fibrosus was applied to the anterior cervical intervertebral of fusion procedure in experimental goats.X-ray films and CT scans were taken after operation to acquire the status of fusional segments.Histological study of CHTF slice were performed to find the differentiation of annulus fibrosus.Result:X-ray films and CT scans showed evidence of stability of the operated segment obtained by CHTF technique.The callus was found around the CHTF after 6 weeks since operation,especially in the contact surface between the endplate of the vertebrae body and metal.Histological study of CHTF filled with cancellous bone showed both new cartillage and bone trabecula formation after 6 weeks since operation.After 12 weeks,new trabecula was obvious and necrosis cancellous was found.Histological study of CHTF filled with cancellous bone and annulus fibrosus showed tissue necrosis and a little fibrous cartilage formation aound the fibrous tissue after 6 weeks since operation.At the point of 12 weeks,new bone trabecula and cartilage were found substituting the old trabecula or fibrous.The CHTF filled only with annulus fibrosus were found both fibrous cartilage and new cartilage formation.The CHTF filled with nothing were only found a little fibrous cartilage formation afer 12 weeks.Conclusion:The study confirms progressive osteogenic capacity of fibroblasts in annular fibrosus in vivo.Perhaps proliferation of chondrocytes could replace normal fibroblast.