| 黄花紫堇、多刺绿绒蒿及其同属近缘物种的ITS2条形码鉴定与分析 |
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| 引用本文： | 豆荣昆,毕振飞,白瑞雪,任瑶瑶,谭睿,宋良科,李迪强,茆灿泉. 黄花紫堇、多刺绿绒蒿及其同属近缘物种的ITS2条形码鉴定与分析[J]. 中国中药杂志, 2015, 40(8): 1453-1458 |
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| 作者姓名： | 豆荣昆 毕振飞 白瑞雪 任瑶瑶 谭睿 宋良科 李迪强 茆灿泉 |
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| 作者单位： | 1. 西南交通大学生命科学与工程学院,四川成都,610031
2. 中国林业科学院森环森保所,北京,100091 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2014XZ09304-307-001-019) |
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| 摘 要： | 运用ITS2 DNA条形码技术鉴定中药材黄花紫堇、多刺绿绒蒿及其近缘物种,以保证药材质量,确保用药安全.文中对采自西藏拉萨的黄花紫堇、多刺绿绒蒿等13个药材样品进行了DNA提取、PCR扩增和测序;对实验序列和GenBank下载的71条切除两端的5.8S,28S区域,使用MEGA 5.0进行多重序列比对,并进行种内、种间遗传距离计算和进化树构建;同时预测了黄花紫堇、多刺绿绒蒿与其近缘种ITS2序列的二级结构.结果表明,作为DNA条形码,ITS2序列能够有效地对黄花紫堇、多刺绿绒蒿及其同属近缘物种进行分子鉴定.该研究不仅可为黄花紫堇、多刺绿绒蒿及其近缘物种、相关混伪品建立基于.ITS2序列的DNA条形码的规范和安全的检测技术及鉴别方法,也为其质量控制、安全用药及合理开发利用提供理论依据及数据支撑.
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| 关 键 词： | 黄花紫堇 多刺绿绒蒿 ITS2序列 DNA条形码 鉴定 |
| 收稿时间： | 2014-10-22 |
Identification and analysis of Corydalis boweri,Meconopsis horridula and their close related species of the same genus by using ITS2 DNA barcode |
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| DOU Rong-kun,BI Zhen-fei,BAI Rui-xue,REN Yao-yao,TAN Rui,SONG Liang-ke,LI Di-qiang and MAO Can-quan. Identification and analysis of Corydalis boweri,Meconopsis horridula and their close related species of the same genus by using ITS2 DNA barcode[J]. China Journal of Chinese Materia Medica, 2015, 40(8): 1453-1458 |
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| Authors: | DOU Rong-kun BI Zhen-fei BAI Rui-xue REN Yao-yao TAN Rui SONG Liang-ke LI Di-qiang MAO Can-quan |
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| Affiliation: | School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu 610031, China,School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu 610031, China,School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu 610031, China,School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu 610031, China,School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu 610031, China,School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu 610031, China,Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China and School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu 610031, China |
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| Abstract: | The study is aimed to ensure the quality and safety of medicinal plants by using ITS2 DNA barcode technology to identify Corydalis boweri,Meconopsis horridula and their close related species. The DNA of 13 herb samples including C. boweri and M. horridula from Lhasa of Tibet was extracted, ITS PCR were amplified and sequenced. Both assembled and web downloaded 71 ITS2 sequences were removed of 5.8S and 28S. Multiple sequence alignment was completed and the intraspecific and interspecific genetic distances were calculated by MEGA 5.0, while the neighbor-joining phylogenetic trees were constructed. We also predicted the ITS2 secondary structure of C. boweri,M. horridula and their close related species. The results showed that ITS2 as DNA barcode was able to identify C. boweri,M. horridula as well as well as their close related species effectively. The established based on ITS2 barcode method provides the regular and safe detection technology for identification of C. boweri, M. horridula and their close related species, adulterants and counterfeits, in order to ensure their quality control, safe medication, reasonable development and utilization. |
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| Keywords: | Corydalis boweri Meconopsis horridula ITS2 DNA barcode identification |
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