Characterization of recombinant malarial RecQ DNA helicase |
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| Authors: | Pattra Suntornthiticharoen Witsanu Srila Porntip Chavalitshewinkoon-Petmitr Paviga Limudomporn Montarop Yamabhai |
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| Affiliation: | 1. Faculty of Science, Rangsit University, Thailand;2. Molecular Biotechnology Laboratory, Suranaree University of Technology, Thailand;3. Faculty of Tropical Medicine, Mahidol University, Thailand |
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| Abstract: | RecQ DNA gene of multi-drug resistant Plasmodium falciparum K1 (PfRecQ1) was cloned, and the recombinant C-terminal-decahistidine-tagged PfRecQ1 was expressed in Escherichia coli. The purified enzyme could efficiently unwind partial duplex DNA substrate in a 3′ to 5′ direction. The malarial RecQ1 could not unwind substrates with both 5′ and 3′ overhangs, those with a 5′ overhang, or blunt-ended DNA duplexes. Unwinding of DNA helicase activity was driven by the hydrolysis of ATP. The drug inhibitory effects of six compounds indicated that only doxorubicin and daunorubicin could inhibit the unwinding activity. |
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| Keywords: | Malaria Plasmodium falciparum DNA helicase RecQ Cloning Expression |
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