淋球菌对抗生素耐药性研究

目的:研究广州地区2001年度分离的淋球菌对青霉素、四环素、壮观霉素、头孢曲松和环丙沙星的耐药性。分析耐药菌株的流行特点。方法:采用琼脂稀释法测定菌株对5种抗生素的最小抑菌浓度(MIC),判断敏感性按WHO西太区淋球菌耐药性监测统一标准。用纸片酸度法检测产青霉素酶淋球菌(PPNG)菌株。结果:153株淋球菌中检出127株对青霉素耐药(83.0％),产青霉素酶淋球菌(PPNG)占17.0％(26/153);四环素耐药129株(84.3％);其中质粒介导高度耐四环素淋球菌菌株(TRNG)占总数的24.1％(37/153);环丙沙星耐药117株(76.5％),其中92株同时对青霉素和四环素有耐药性,三重耐药占60.1％;壮观霉素和头孢曲松敏感性最高,分别为100.0％和81.0％。结论:淋球菌对青霉素、四环素和环丙沙星耐药率较高;壮观霉素和头孢曲松敏感,应作为临床的首选药物。

Study on Antibiotic Resistant of Neisseria Gonorrhoeae

Objective:To investigate the antimicrobial susceptibiity and the resistant trend of Neisse-ria gionorrnoeae strains isolated in Guangzhou in year of 2001. Methods: The agar dilution method was used to determine the minimum inhibitory concentrations ( MICs) for penicillin G, tetracycline, ciprofloxcin, ceftriaxone and spetinomycin. The resistance of all strains to five antibiotics were interpreted according to criteria used in the project of surveillance of gonococcal antibiotic susceptibility in WHO Western Pacific Region. Penicillinase - producing N. gonorrhoeae (PPNG) was analyzed by the paper acidometric method. Results: 153 consecutive N. gonorrhoeae isolates collected in Guangzhou were studied in year of 2001. Penicillin - resistant rate was 83.0% (127/153) and PPNG was 17.0% (26/153). 84.3% (129/153) of tetracycline - resistant isolates were idenified and high level tetracycline - resistant(TRNG) accounted for 24.1 % (37/153) . The prevalence of ciprofloxacin - resistance was 76.5%(117/153), in which 92 (60.1% ) strains showed multi - resistant to penicillin and tetracycline. Spectinomycin and ceftriaxone appeared to be 100% and 81.0% in sensitivity respectively. Conclusion: Resistance to penicillin, tetracycline and ciprofloxacin spread seriously in Guangzhou. Ceftriaxone and spectionycin should be used as the first - line agents in treatment of gonrrhoea.