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Evidence for A1 and A3 receptors mediating adenosine-induced intracellular calcium release in the dorsal root ganglion neurons by using confocal microscopy imaging |
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| Authors: | Liqin Zheng Jiangxu Chen Yimei Huang Yuhua Wang Hongqin Yang Yanding Zhang Shusen Xie |
| Affiliation: | 1. Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Collage of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou, 350007, China 2. Fujian Key Laboratory of Developmental and Neural Biology, College of Life Sciences, Fujian Normal University, Fuzhou, 350108, China |
| Abstract: | Adenosine exerts a key role in analgesia. In the present study, adenosine-induced Ca2+ responses were revealed by using confocal microscopy imaging in the rat dorsal root ganglia (DRG) neurons in vitro. Our results showed that adenosine could evoke increases in the intracellular Ca2+ concentration in the DRG neurons. In addition, by application of selective receptor antagonists, two types of receptors, A1R and A3R, were identified to be involved in the adenosine-induced Ca2+ release from intracellular stores in neurons. Altogether, these results suggest that confocal microscopy imaging combined with fluorescent dyes could help to detect the analgesic-induced ion signaling in single cell. |
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