脑脊液中细胞源性囊泡对间充质干细胞向神经前体细胞分化的影响

目的探讨脑脊液微囊泡在间充质干细胞(MSCs)向神经干细胞分化中的作用。方法从人脑脊液中分离微囊泡,从小鼠骨髓中分离原代MSCs。将MSCs传代后在神经分化诱导培养基和脑脊液微囊泡作用下神经分化诱导。细胞形态学观察细胞突起数目和长度,western blot检测细胞神经特异性蛋白表达水平变化。结果单纯神经诱导液组单位面积内细胞突起长度为(2.2±0.4)mm,突起数量为(94±12)个;神经诱导液加脑脊液微囊泡诱导的细胞中细胞突起更长(5.7±1.2)mm,数目也更多(178.2±32)个,差异具有统计学意义(P0.01)。微囊泡组细胞中NSE、Nestin和MAP-2表达水平高于单纯神经诱导液组细胞,差异具有统计学意义(P0.01)。结论脑脊液微囊泡可有效促进MSCs向神经干细胞分化,有望为干细胞移植治疗神经功能缺损提供了一个新的思路。

Role of cell-derived microparticles in cerebrospinal fluid in differentiation of mesenchymal stem cells into neural stem cells

Objective To investigate the role of cell-derived microparticles (MPs) in the differentiation of mesenchymal stem cells (MSCs) into neural stem cells.Methods MPs were isolated from human cerebrospinal fluid and primary MSCs were isolated from mouse bone marrow. After subculture, neural differentiation of MSCs was induced in a specialized medium with the presence of MPs. The number and length of cell processes were observed, and Western blot was used to measure the change in the expression of neuron-specific proteins.Results Compared with the neural induction medium group, the neural induction medium+MPs group had significantly longer cell processes (5.7±1.2 mm vs 2.2±0.4 mm, P<0.01) and a significantly higher number of cell processes (178.2±32 vs 94±12, P<0.01). The neural induction medium+MPs group had significantly higher expression of NSE, Nestin, and MAP-2 than the neural induction medium group (P<0.01).Conclusions MPs in cerebrospinal fluid can effectively promote the differentiation of MSCs into neural stem cells, which may provide new thoughts for stem cell transplantation in the treatment of neurological defects.