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| 复方中药金芪滴眼剂对兔角膜上皮及基质细胞增殖的影响 | |
| 引用本文： | 王津津,杨永升,关洁,李根林,金秀英. 复方中药金芪滴眼剂对兔角膜上皮及基质细胞增殖的影响[J]. 中国实用眼科杂志, 2003, 21(3): 230-231 |
| 作者姓名： | 王津津 杨永升 关洁 李根林 金秀英 |
| 作者单位： | 1. 首都医科大学附属北京同仁医院,100730 2. 山西省眼科医院 3. 北京眼科研究所 |
| 摘 要： | 目的：观察复方中药金芪滴眼剂对兔角膜上皮细胞及兔角膜基质层细胞增殖的影响。方法：建立体外培养兔角膜上皮细胞，兔角膜基质层细胞系，用MTT法检测不同浓度金芪滴眼剂对细胞增殖活性的影响。结果：金芪滴眼剂对兔角膜上皮细胞有显著的促进增殖作用。且随浓度的增加。增殖率加大，对兔角膜基质层细胞无促增殖作用。高浓度时有抑制作用。结论：复方中药金芪滴眼剂对兔角膜上皮细胞有较好的保护作用。 |
| 关 键 词： | 复方中药 金芪滴眼剂 兔 角膜上皮 基质细胞增殖 角膜基质层细胞 细胞培养 MTT检测 |
| 修稿时间： | 2002-09-01 |
Chinese herbs on the growth of rabbit corneal epithelial cells and corneal stroma cells in vitro |
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| WANG Jinjin,YANG Yongsheng,GUAN JieCentral Laboratory,Beijing Tongren Hospital,Beijing ,China. Chinese herbs on the growth of rabbit corneal epithelial cells and corneal stroma cells in vitro[J]. Chinese Journal of Practical Ophthalmology, 2003, 21(3): 230-231 | |
| Authors: | WANG Jinjin YANG Yongsheng GUAN JieCentral Laboratory Beijing Tongren Hospital Beijing China |
| Affiliation: | WANG Jinjin,YANG Yongsheng,GUAN JieCentral Laboratory,Beijing Tongren Hospital,Beijing 100730,China |
| Abstract: | Objective:To observe the impacts of JINQI,a kind of compound of Chinese herbs,on the growth of rabbit corneal epithelial cells and corneal stroma cells in vitro. Methods:Corneal epithelial cells and stroma cells were cultured with different doses of JINQI for 24 hours,then the proliferation rate was detected by MTT method. Results:JINQI could promote the proliferation of corneal epithelial cells in dose-dependent manner,but not of corneal stroma cells.And,JINQI in high dose could inhibit the growth of stroma cells. Conclusions:Eye drops of JINQI promote the proliferation of rabbit corneal epithelial cells effectively. |
| Keywords: | Chinese herbs Corneal epithelial cell Corneal stroma Cell culture MTT assay |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |