增生性瘢痕和瘢痕疙瘩中PKC活性测定

目的　测定增生性瘢痕和瘢痕疙瘩等瘢痕组织中PKC活性变化 ,探讨PKC在瘢痕形成中的作用。方法　利用PKC活化后催化底物多肽磷酸化 ,然后计数底物中掺入32 P的放射活性的原理 ,测定增生性瘢痕、瘢痕疙瘩、成熟瘢痕和正常皮肤组织中PKC活性。结果　增生性瘢痕和瘢痕疙瘩组织中PKC的活性显著高于成熟瘢痕和正常皮肤 (P <0 .0 0 1,分别高出正常皮肤 318.30 %和 5 19.0 7% ) ,瘢痕疙瘩高于增生性瘢痕 (P <0 .0 0 1,高出 48.6 0 % )而成熟瘢痕和正常皮肤间没有差异 (P >0 .0 5 )。结论　瘢痕组织中PKC活性升高且与增生程度相关 ,提示PKC可能参与了细胞增殖和合成物质的信号转导

Activity of protein kinase C in hypertrophic scar and keloid tissue

Objective To determine the activity of protein kinase C(PKC) in hypertrophic scar(HS) and keloid(K) tissue and to study the effects of PKC on scar formation. Methods The principle of activated-PKC to transfer phosphorus ( 32 P) into substrate was used to calculate activity of PKC by detecting radioactivity of substrate with scintillation counter. Six cases of HS and K were studied. Mature scar (MS) and normal skin (NS) were used as control. Results Activity of PKC in HS and K was obviously elevated as compared with that in MS and NS (P<0.001): and in keloid its activity was higher than in prolifenative scar. The difference between MS and NS was insignificant (P>0.05). Conclusion Activity of PKC in scar tissue is elevated and increasing degree of PKC may be related to proliferative level of scar.