|
|||||
|
|
| 应用PCR技术诊断单纯疱疹病毒性角膜炎 | |
| 引用本文： | 朱其新,余沃莲. 应用PCR技术诊断单纯疱疹病毒性角膜炎[J]. 中国热带医学, 2005, 5(6): 1242-1242,1244 |
| 作者姓名： | 朱其新 余沃莲 |
| 作者单位： | 肇庆市第二人民医院,广东,肇庆,526060 |
| 摘 要： | 目的探讨聚合酶链反应（PCR）技术诊断单纯疱疹（单疱）病毒性角膜炎的实用价值，为临床诊断提供依据和方法。方法应用PCR技术对60例按临床诊断标准诊断为单疱病毒性角膜炎患者的患眼泪液及角膜上皮进行病毒DNA检测。结果PCR检测60例患者标本结果，阳性46例占76．7％，阴性14例占23．3％。结论对单疱病毒性角膜炎诊断不能只根据其临床特征来诊断；PCR可为临床提供实验诊断依据。 |
| 关 键 词： | 单疱病毒 角膜炎 泪液和角膜上皮 聚合酶链反应（PCR） |
| 文章编号： | 1009-9727（2005）06-1242-01 |
| 收稿时间： | 2005-06-08 |
| 修稿时间： | 2005-06-08 |
Diagnosis of herpes simplex keratitis by using PCR |
|
| ZHU Qi-xin,YU Wo-lian. Diagnosis of herpes simplex keratitis by using PCR[J]. China Tropical Medicine, 2005, 5(6): 1242-1242,1244 | |
| Authors: | ZHU Qi-xin YU Wo-lian |
| Abstract: | Objective To detect herpes simplex virus (HSV) in tear and corneal epithelium of patients with herpes simlex keratitis (HSK). Methods Tear and corneal epithelium of 60 cases clinically diagnosed as HSK were tested by polymerase chain reaction (including 24 cases of punctate keratopathy, 29 cases of map keratopathy and 7 cases of disciform keratopathy). Results The result was positive in 46 cases (76.7%) and negative in 14 (23.3%). Conclusion Polymerase chain reaction is an experimental approach to diagnose HSK. |
| Keywords: | Herpes simplex virus Keratitis Tears and corneal epithelium Polymerase chain reaction(PCR) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |