胰岛素样生长因子-1抗氧化损伤雪旺细胞凋亡的实验研究

目的探讨胰岛素样生长因子-1（IGF-1）对H2O2损伤的雪旺细胞（SCs）抗凋亡的作用。方法将培养的SCs分为H₂O₂处理组、H₂O₂加IGF-1保护组和正常对照组。检测各组细胞内超氧化物歧化酶（SOD）及丙二醛(MDA)的含量，丫叮橙（AO）染色观察细胞存活、凋亡情况，蛋白印迹（Western blot）法检测Bcl-2的表达水平。结果与正常组及保护组相比，H₂O₂处理组细胞具有典型的凋亡形态特征：核染色质浓染、边集，细胞体积缩小，细胞存活率降低；SOD含量明显减少（P＜0.01)，MDA含量明显增加（P＜0.01)，Bcl-2表达下调；而IGF-1保护组细胞存活率明显升高（P＜0.01)，SOD含量较损伤组增高（P＜0.01)，MDA含量较损伤组明显减少（P＜0.01)，Bcl-2表达明显上调。结论本实验结果提示IGF-1对氧化损伤的SCs具有保护作用。

Anti-apoptosis effect of insulin-like growth factor-1 on oxidative injured Schwann cells

Objective To investigate the anti-apoptosis effects of insulin-like growth factor-1 on oxidative injured Schwann cells(SCs).Methods Cultured SCs of rats were divided into the H2O2 group,the H2O2 and IGF-1 group,and the control group.The amounts of intracellular superoxide dismutase(hepatocuprein,SOD) and malondialdehyde(malonaldehyde,MDA)were determined by a biological chemical method,apoptosis was determined by acridine orange staining,and Bcl-2 expression was determined by Western blot.Results Activities of SCs in the oxidative injured group decreased compared with the control group.However,these changes were significantly reversed by the IGF-1 pre-conditioning.Conclusion IGF-1 has an anti-apoptosis effect on oxidative injured SCs.