大鼠肾脏细胞17β-HSD1的表达及参与性激素合成的能力

目的通过研究合成性激素的关键酶17β-HSD1在肾脏中的表达，探讨肾脏是否具备合成性激素的作用。方法基于无促
卵泡生成素（FSH）、黄体生成素（LH）培养基和有FSH、LH培养基两种条件下，Western blotting、放射免疫分析法分别检测培
养24、48 h 后肾脏细胞中17β-HSD1 的表达和性激素的分泌情况。结果培养24 h 后，大鼠肾脏细胞能够表达少量的
17β-HSD1 蛋白（0.1843±0.076），同时能够分泌少量的雌二醇、孕酮和睾酮（分别为3.30±3.78 nmol/L，62.60±12.33 pmol/L 和
22.12±3.36 nmol/L），而在FSH和LH的共同刺激下，大鼠肾脏细胞17β-HSD1蛋白的表达量明显升高（1.6651±0.044，P<0.01），
同时分泌雌二醇、孕酮和睾酮的量也显著增加（分别为8.50±2.64 nmol/L，117.80±9.79 pmol/L和45.04±4.39 nmol/L，均P<0.05），
培养24 h和48 h上述指标均无明显差异（P>0.05）。结论大鼠肾脏细胞中有17β-HSD1的表达，并且在FSH和LH的共刺激下
能够稳定分泌性激素，提示肾脏组织具备合成性激素的能力，丰富了肾脏的内分泌功能。

Expression of 17 beta-hydroxysteroid dehydrogenase type 1 in the kidney of rats: the capacity of the kidney for synthesizing sex hormones

Objective To investigate the expression of 17 beta-hydroxysteroid dehydrogenase type 1 (17β-HSD1) in the kidney of
rats and explore the capacity of the kidney for synthesizing sex hormones. Methods The expressions of 17-HSD1 and sex
hormones were detected by Western blotting and radioimmunoassay in rat renal cells in primary cultured for 24 and 48 h in
the presence or absence of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Results After cell culture for 24
h, the primary rat renal cells expressed a low level of 17β-HSD1 (0.1843±0.076), which increased to 1.6651±0.044 (P<0.01) in
response to co-stimulation by FSH and LH. Low levels of estradiol, progesterone and testosterone were also detected in rat
renal cells (3.30±3.78, 62.60±12.33, and 22.12±3.36, respectively), and co-stimulation of FSH and LH significantly increased their
levels to 8.50 ± 2.64, 117.80 ± 9.79, and 45.04 ± 4.39, respectively (P<0.05). The levels of these hormones showed no significant
differences between cells cultured for 24 h and 48 h (P>0.05). Conclusion The rat renal cells express 17β-HSD1 and are capable
of stably secreting sex hormones in response to co-stimulation with FSH and LH, suggesting the capacity of the rat kidneys for
synthesizing sex hormones. These findings enrich the understanding of the endocrine function of the kidney.