乳化氟碳联合川芎嗪对大鼠肺缺血再灌注损伤的保护作用

目的探讨乳化氟碳联合川芎嗪对肺缺血再灌注损伤的影响及其机制。方法建立大鼠肺缺血再灌注模型，随机分为对照
组（C组）、川芎嗪组（T组）、乳化氟碳组（P组）和乳化氟碳联合川芎嗪组（TP组），每组10只，分别在恢复血供前5 min由尾静脉给
药，并在恢复血供3 h后处死动物，取肺脏组织测定丙二醛（MDA）、髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、肿瘤坏死因
子-α（TNF-α）含量，观察肺脏组织病理改变并评分。结果T组、P组、TP组肺组织MDA、MPO含量显著低于C组（P<0.05），TP
组MPO含量显著低于T组和P组（P<0.05）；T组、P组、TP组肺组织SOD含量显著高于C组（P<0.05），TP组SOD活性显著高T
组和P组（P<0.05）；4组动物肺组织TNF-α含量无统计学差异（P>0.05）；C组肺组织可见明显水肿、肺泡腔可见红细胞及渗出；T
组、P组肺组织未见明显水肿，可见少量红细胞及渗出；TP组肺组织未见明显水肿，可见少量渗出；C组病理积分大于T组、P组及
TP组，T组及P组病理积分大于TP组（P<0.05）。结论乳化氟碳及川芎嗪可提高内源性清除氧自由基的能力，抑制中性粒细胞
在肺内聚集，减少肺缺血再灌注损伤，两者联合使用效果更好。

Protective effects of perfluorocarbon combined with ligustrazine against lung ischemia-reperfusion injury in rats

Objective To investigate the effects of perfluorocarbon and ligustrazine in protecting the lungs against
ischemia-reperfusion injury in rats. Methds Forty SD rats with ischemia-reperfusion lung injury were randomized equally into
control, ligustrazine, perfluorocarbon, and perfluorocarbon plus ligustrazine groups and received the corresponding treatment
via the tail vein 5 min before reperfusion. The lung tissues were harvested and the levels of malondialdehyde (MDA),
myeloperoxidase (MPO), superoxide dismutase (SOD) and tumor necrosis factor-α (TNF-α) were detected 3 h after
reperfusion. The pathological changes and pathological scores of the lung tissues were analyzed. Results MDA and MPO
levels were significantly lower and SOD activities significantly higher in the lung tissues in the 3 treatment groups than in the
control group (P<0.05). The rats in the combined treatment group showed a significantly lower MPO level and a significantly
higher SOD activity than those treated with ligustrazine or perfluorocarbon alone (P<0.05). No significant difference was
found in TNF-α levels in the lung tissues among the 4 groups (P>0.05). The lung tissues in the control group showed obvious
edema and exudation, and the tissues in ligustrazine and perfluorocarbon groups showed no edema but with a few red blood
cells and exudation; no edema was found in the combined treatment group with only a small amount of exudation. The
pathological scores differed significantly among the 4 groups. Conclusion Perfluorocarbon and ligustrazine, especially in
combined use, can promote endogenous oxygen free radical scavenging, decrease peripheral blood proinflammatory
cytokines, and inhibit neutrophils filtration in the lungs of rats with ischemia/reperfusion lung injury.